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Literature summary for 6.3.4.16 extracted from
- Carbamoylphosphate synthetase I of rat-liver mitochondria. Purification, properties, and polypeptide molecular weight (1978), Eur. J. Biochem., 85, 373-383.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetylglutamate | activates | Rattus norvegicus |  |
| acetylglutamate | Km: 0.1 mM | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Amino-4-oxo-5-chloropentanoic acid | - |
Rattus norvegicus | |
| Mn2+ | activates | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
ATP | MgATP2- | Rattus norvegicus | |
| 2 | - |
NH4+ | - |
Rattus norvegicus | |
| 5 | - |
HCO3- | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Rattus norvegicus | |
| Mg2+ | Mg2+ and Mn2+ are equally effective activators in the direction of carbamoyl phosphate synthesis, Km: 0.17 mM | Rattus norvegicus | |
| Mn2+ | Mg2+ and Mn2+ are equally effective activators in the direction of carbamoyl phosphate synthesis. Excess Mn2+ inhibits | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 158000 | - |
1 * 158000, sedimentation equilibrium in 6 M guanidine hydrochloride containing DTT, predominantly exists in the monomeric form | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ATP + NH4+ + HCO3- | - |
Rattus norvegicus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
| 2 ATP + NH4+ + HCO3- | ATP in form of MnATP2- | Rattus norvegicus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
| ATP + NH4+ + CO2 + H2O | no activity with Gln | Rattus norvegicus | ADP + phosphate + carbamoylphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 158000, sedimentation equilibrium in 6 M guanidine hydrochloride containing DTT, predominantly exists in the monomeric form | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.6 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 8 | 50% of maximal activity at pH 6.3 and 8.0 | Rattus norvegicus |
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Release 2023.1 (January 2023)
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