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Literature summary for 6.3.4.16 extracted from

  • Eroglu, B.; Powers-Lee, S.G.
    Unmasking a functional allosteric domain in an allosterically nonresponsive carbamoyl-phosphate synthetase (2002), J. Biol. Chem., 277, 45466-45472.
    View publication on PubMed
Search for more literature for 6.3.4.16

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D265A no carbamoyl phosphate synthesis Saccharomyces cerevisiae
D265E about 10% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae
D265N about 10% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae
D810A no carbamoyl phosphate synthesis Saccharomyces cerevisiae
D810E no carbamoyl phosphate synthesis Saccharomyces cerevisiae
D810N no carbamoyl phosphate synthesis Saccharomyces cerevisiae
H262N no carbamoyl phosphate synthesis Saccharomyces cerevisiae
H807N about 30% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae
I316G about 10% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae
I316H no carbamoyl phosphate synthesis Saccharomyces cerevisiae
I316S about 30% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae
L229G about 30% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae
S228A about 30% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters for utilization of ATP and ADP by mutant enzymes Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 LPL26
-
Saccharomyces cerevisiae LPL26
-
 LPL26
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
 Saccharomyces cerevisiae 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
2 ATP + L-Gln + HCO3-
-
 Saccharomyces cerevisiae LPL26 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
2 ATP + NH4+ + HCO3-
-
 Saccharomyces cerevisiae 2 ADP + phosphate + carbamoyl phosphate
-
 ?
2 ATP + NH4+ + HCO3-
-
 Saccharomyces cerevisiae LPL26 2 ADP + phosphate + carbamoyl phosphate
-
 ?