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Literature summary for 6.3.4.16 extracted from

  • Saeed-Kothe, A.; Powers-Lee, S.G.
    Gain of glutaminase function in mutants of the ammonia-specific frog carbamoyl phosphate synthetase (2003), J. Biol. Chem., 278, 26722-26726.
    View publication on PubMed
Search for more literature for 6.3.4.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces pombe Lithobates catesbeianus

Protein Variants

Protein Variants Comment Organism
E261Q no synthesis of carbonyl phosphate in presence of L-glutamine Lithobates catesbeianus
K258L synthesis of carbonyl phosphate in presence of L-glutamine Lithobates catesbeianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 ATP wild-type enzyme, comparison to native and mutant enzyme Lithobates catesbeianus
0.39
-
 ammonia wild-type enzyme, comparison to native and mutant enzyme Lithobates catesbeianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ATP + NH4+ + HCO3- Homo sapiens
-
 2 ADP + phosphate + carbamoyl phosphate
-
 ?
2 ATP + NH4+ + HCO3- Rattus norvegicus
-
 2 ADP + phosphate + carbamoyl phosphate
-
 ?
2 ATP + NH4+ + HCO3- Lithobates catesbeianus
-
 2 ADP + phosphate + carbamoyl phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Lithobates catesbeianus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lithobates catesbeianus

Reaction

Reaction Comment Organism Reaction ID
2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate depending on their physiological role, carbonyl phosphate synthetases use either glutamine or free ammonia as the nitrogen donor for carbamoyl phosphate synthesis, all enzymes contain the structurally equivalent of a triad-type glutamine amidotransferase, GAT, domain Lithobates catesbeianus
a
2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate depending on their physiological role, carbonyl phosphate synthetases uses either glutamine or free ammonia as the nitrogen donor for carbamoyl phosphate synthesis, all enzymes contain the structurell equivalent of a triad-type glutamine amidotransferase, GAT, domain Homo sapiens
a
2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate depending on their physiological role, carbonyl phosphate synthetasese uses either glutamine or free ammonia as the nitrogen donor for carbamoyl phosphate synthesis, all enzymes contain the structurell equivalent of a triad-type glutamine amidotransferase, GAT, domain Rattus norvegicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + NH4+ + HCO3-
-
 Homo sapiens 2 ADP + phosphate + carbamoyl phosphate
-
 ?
2 ATP + NH4+ + HCO3-
-
 Rattus norvegicus 2 ADP + phosphate + carbamoyl phosphate
-
 ?
2 ATP + NH4+ + HCO3-
-
 Lithobates catesbeianus 2 ADP + phosphate + carbamoyl phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 ammonia wild-type enzyme, comparison to native and mutant enzyme Lithobates catesbeianus
2.4
-
 ATP wild-type enzyme, comparison to native and mutant enzyme Lithobates catesbeianus