Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a mutation study reveals that Escherichia coli TilS discriminates tRNAIle from the structurally similar tRNAMet having the same anticodon loop by recognizing the anticodon loop, the anticodon stem, and the acceptor stem | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters of Escherichia coli TilS with various tRNAIle2 mutants and with tRNAMet mutants | Escherichia coli | |
0.00038 | - |
[tRNAIle2]-cytidine34 | unmodified tRNAIle2, pH 7.8, 37°C | Escherichia coli | |
0.22 | - |
ATP | pH 7.8, 37°C | Escherichia coli | |
0.38 | - |
L-lysine | pH 7.8, 37°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[tRNAIle2]-cytidine34 + L-lysine + ATP | Escherichia coli | - |
[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[tRNAIle2]-cytidine34 + L-lysine + ATP | - |
Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? | |
[tRNAIle2]-cytidine34 + L-lysine + ATP | lysidine synthesis consists of two consecutive reactions that involve an adenylated tRNA intermediate. A mutation study reveals that Escherichia coli TilS discriminates tRNAIle from the structurally similar tRNAMet having the same anticodon loop by recognizing the anticodon loop, the anticodon stem, and the acceptor stem. TilS binds to the anticodon region and 3' side of the acceptor stem, which cover the recognition sites. A dedicated mechanism is embedded in tRNAIle that controls its recognition and discrimination by TilS | Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TilS | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters of Escherichia coli TilS with various tRNAIle2 mutants and with tRNAMet mutants | Escherichia coli | |
0.012 | - |
[tRNAIle2]-cytidine34 | unmodified tRNAIle2, pH 7.8, 37°C | Escherichia coli | |
0.015 | - |
ATP | pH 7.8, 37°C | Escherichia coli | |
0.07 | - |
L-lysine | pH 7.8, 37°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | 2-lysyl cytidine is a lysine-containing cytidine derivative commonly found at the wobble position of bacterial AUA codon-specific tRNAIle. This modification determines both codon and amino acid specificities of tRNAIle | Escherichia coli |