Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.4.19 extracted from

  • Koehrer, C.; Mandal, D.; Gaston, K.W.; Grosjean, H.; Limbach, P.A.; Rajbhandary, U.L.
    Life without tRNAIle-lysidine synthetase: translation of the isoleucine codon AUA in Bacillus subtilis lacking the canonical tRNA2Ile (2014), Nucleic Acids Res., 42, 1904-1915.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.4.19

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[tRNAIle2]-cytidine34 + L-lysine + ATP Bacillus subtilis
-
 [tRNAIle2]-lysidine34 + AMP + diphosphate + H2O
-
 ?
[tRNAIle2]-cytidine34 + L-lysine + ATP Bacillus subtilis 168
-
 [tRNAIle2]-lysidine34 + AMP + diphosphate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 168
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[tRNAIle2]-cytidine34 + L-lysine + ATP
-
 Bacillus subtilis [tRNAIle2]-lysidine34 + AMP + diphosphate + H2O
-
 ?
[tRNAIle2]-cytidine34 + L-lysine + ATP
-
 Bacillus subtilis 168 [tRNAIle2]-lysidine34 + AMP + diphosphate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
TilS
-
 Bacillus subtilis
tRNAIle-lysidine synthetase
-
 Bacillus subtilis

General Information

General Information Comment Organism
malfunction the absence of enzyme activity leads to a lack of modification of cytidine34 to lysidine in tRNA2IleLAU, (i.e. synthesis of tRNA2IleCAU), thereby rendering it inactive for translation of AUA codons. Strains of Bacillus subtilis carrying a deletion in the TilS gene can survive only in the presence of a mutant tRNA1Ile, whose anticodon wobble position is changed from G34 to U34 (tRNA1IleUAU) Bacillus subtilis