Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.4.2 extracted from

  • Kizaki, H.; Sakurada, T.; Weber, G.
    Purification and properties of CTP synthetase from Ehrlich ascites tumor cells (1981), Biochim. Biophys. Acta, 662, 48-54.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol required for optimal activity, above 50 mM increase activity to 221% Mus musculus
dithiothreitol required for optimal activity, above 50 mM increase activity to 223% mM Mus musculus
GTP not essential, but acts as activator on the glutamine reaction, optimal activation at 1 mM Mus musculus

General Stability

General Stability Organism
2-mercaptoethanol is required for optimal stabilization of enzyme activity Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
PCMB 0.01 mM, 50% inhibition Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, no activity in absence of Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000
-
gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
Ehrlich ascites carcinoma cell
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
-
Mus musculus

Storage Stability

Storage Stability Organism
-20°C, 30% glycerol, 100 mM 2-mercaptoethanol, 40% loss of activity after 4 weeks Mus musculus
-80°C, 30% glycerol, 100 mM 2-mercaptoethanol, 65% of the original activity is retained after 4 weeks Mus musculus
4°C, 30% glycerol, 100 mM 2-mercaptoethanol, 65% loss of activity after 4 weeks Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UTP + glutamine
-
Mus musculus ADP + phosphate + CTP + Glu
-
?
ATP + UTP + NH4+
-
Mus musculus ADP + phosphate + CTP
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
reaction with ammonia Mus musculus
8.6 8.9 reaction with glutamine Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
7.3 9.5 pH 7.3: about 35% of maximal activity, pH 9.5: about 40% of maximal activity Mus musculus