Protein Variants | Comment | Organism |
---|---|---|
H125S | site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion | Clostridium cylindrosporum |
H131S | site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion | Clostridium cylindrosporum |
H268S | site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion | Clostridium cylindrosporum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
ADP | tetrahydrofolate, mutant enzyme K71Q | Clostridium cylindrosporum | |
0.23 | - |
tetrahydrofolate | MgATP2-, wild-type enzyme | Clostridium cylindrosporum | |
0.26 | - |
ATP | mutant enzyme K71Q | Clostridium cylindrosporum | |
0.4 | - |
ATP | mutant enzyme H131S | Clostridium cylindrosporum | |
0.59 | - |
ATP | mutant enzyme H125S | Clostridium cylindrosporum | |
0.69 | - |
tetrahydrofolate | wild-type enzyme | Clostridium cylindrosporum | |
0.97 | - |
tetrahydrofolate | mutant enzyme H125S | Clostridium cylindrosporum | |
1.12 | - |
tetrahydrofolate | mutant enzyme H131S | Clostridium cylindrosporum | |
6.7 | - |
formate | wild-type enzyme | Clostridium cylindrosporum | |
8.2 | - |
formate | mutant enzyme H125S | Clostridium cylindrosporum | |
12.1 | - |
formate | mutant enzyme K71Q | Clostridium cylindrosporum | |
20.2 | - |
formate | mutant enzyme H131S | Clostridium cylindrosporum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium cylindrosporum | - |
wild-type and site-directed mutant enzymes H125S, H131S, and H268S | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + formate + tetrahydrofolate | - |
Clostridium cylindrosporum | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
ATP + formate + tetrahydrofolate | ATP in form of MgATP2- | Clostridium cylindrosporum | ADP + phosphate + 10-formyltetrahydrofolate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Clostridium cylindrosporum |