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Literature summary for 6.3.4.3 extracted from

  • Kounga, K.; Song, S.; Haslam, G.C.; Himes, R.H.
    Site-directed mutagenesis of putative catalytic and nucleotide binding sites in N10-formyltetrahydrofolate synthetase (1996), Biochim. Biophys. Acta, 1296, 112-130.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H125S site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion Clostridium cylindrosporum
H131S site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion Clostridium cylindrosporum
H268S site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion Clostridium cylindrosporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
ADP tetrahydrofolate, mutant enzyme K71Q Clostridium cylindrosporum
0.23
-
tetrahydrofolate MgATP2-, wild-type enzyme Clostridium cylindrosporum
0.26
-
ATP mutant enzyme K71Q Clostridium cylindrosporum
0.4
-
ATP mutant enzyme H131S Clostridium cylindrosporum
0.59
-
ATP mutant enzyme H125S Clostridium cylindrosporum
0.69
-
tetrahydrofolate wild-type enzyme Clostridium cylindrosporum
0.97
-
tetrahydrofolate mutant enzyme H125S Clostridium cylindrosporum
1.12
-
tetrahydrofolate mutant enzyme H131S Clostridium cylindrosporum
6.7
-
formate wild-type enzyme Clostridium cylindrosporum
8.2
-
formate mutant enzyme H125S Clostridium cylindrosporum
12.1
-
formate mutant enzyme K71Q Clostridium cylindrosporum
20.2
-
formate mutant enzyme H131S Clostridium cylindrosporum

Organism

Organism UniProt Comment Textmining
Clostridium cylindrosporum
-
wild-type and site-directed mutant enzymes H125S, H131S, and H268S
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + formate + tetrahydrofolate
-
Clostridium cylindrosporum ADP + phosphate + 10-formyltetrahydrofolate
-
?
ATP + formate + tetrahydrofolate ATP in form of MgATP2- Clostridium cylindrosporum ADP + phosphate + 10-formyltetrahydrofolate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Clostridium cylindrosporum