Protein Variants | Comment | Organism |
---|---|---|
additional information | in Escherichia coli DELTAfolD mutants, the essential function of folD can be replaced by Clostridium perfringens fhs when it is provided on a medium-copy-number plasmid or integrated as a single-copy gene in the chromosome. The fhs-supported folD deletion (DELTAfolD) strain grows well in a complex medium. The DELTAfolD strain, harboring heterologous fhs on the chromosome, shows a high NADP+ -to-NADPH ratio and hypersensitivity to trimethoprim. The presence of fhs in Escherichia coli is disadvantageous for its aerobic growth. but under hypoxia, Escherichia coli strains harboring fhs outcompete those lacking it | Clostridium perfringens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + formate + tetrahydrofolate | Clostridium perfringens | - |
ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
ATP + formate + tetrahydrofolate | Clostridium perfringens DSM 756 | - |
ADP + phosphate + 10-formyltetrahydrofolate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | Q0TMI3 | - |
- |
Clostridium perfringens DSM 756 | Q0TMI3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + formate + tetrahydrofolate | - |
Clostridium perfringens | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
ATP + formate + tetrahydrofolate | - |
Clostridium perfringens DSM 756 | ADP + phosphate + 10-formyltetrahydrofolate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FHS | - |
Clostridium perfringens |
Formyltetrahydrofolate synthetase | - |
Clostridium perfringens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Clostridium perfringens |
General Information | Comment | Organism |
---|---|---|
evolution | one-carbon metabolic pathway rewiring in Escherichia coli reveals an evolutionary advantage of 10-formyltetrahydrofolate synthetase in survival under hypoxia | Clostridium perfringens |
physiological function | N10-formyltetrahydrofolate is required for formylation of eubacterial/organellar initiator tRNA and purine nucleotide biosynthesis. Biosynthesis of N10-formyltetrahydrofolate is catalyzed by 5,10-methylene-tetrahydrofolate dehydrogenase/cyclohydrolase (FolD, EC 1.5.1.3) and/or 10-formyltetrahydrofolate synthetase (Fhs) | Clostridium perfringens |