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Literature summary for 6.3.4.3 extracted from
- NAD-dependent methylenetetrahydrofolate dehydrogenase inhibits oral squamous cell carcinoma cell proliferation and promotes apoptosis (2021), Transl. Cancer Res., 10, 1457-1469 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | MTHFD1L may be involved in OSCC progression via the c-MYC gene and p53 signaling and may serve as a target and orientation for tumor therapy | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | short hairpin RNA (shRNA)-induced MTHFD1L silencing, analysis of the effcets on the biological behavior of OSCC cells are assessed in vitro and in vivo, overview. Knockdown of MTHFD1L suppresses cell proliferation, colony formation, and tumorigenesis, while it induces apoptosis in oral squamous cell carcinoma (OSCC). Mechanistically, a microarray analysis shows that MTHFD1L suppresses c-MYC and activates p53 signaling by regulating the protein expression of TP53, GADD45A, FAS and JUN | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | MTHFD1L is a mitochondrial monofunctional enzyme with 10-CHO-THF synthase activity | Homo sapiens | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + formate + tetrahydrofolate | Homo sapiens | - |
ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| additional information | Homo sapiens | MTHFD1L directly interacts with the c-MYC proto-oncogene and basic helix-loop-helix (bHLH) transcription factor. MTHFD1L directly interacts with the MAGE family member D1 (MAGED) | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q6UB35 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | MTHFD1L levels are higher in tumor tissue than in adjacent tissues, RNA analysis and immunohistochemic analysis, overview | Homo sapiens | - |
| oral squamous cell carcinoma cell | OSCC cell. The p53 pathway might contribute to the abnormal expression of MTHFD1L in OSCC | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + formate + tetrahydrofolate | - |
Homo sapiens | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
| additional information | MTHFD1L directly interacts with the c-MYC proto-oncogene and basic helix-loop-helix (bHLH) transcription factor. MTHFD1L directly interacts with the MAGE family member D1 (MAGED) | Homo sapiens | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 10-CHO-THF synthase | - |
Homo sapiens |
| methylenetetrahydrofolate dehydrogenase 1-like | - |
Homo sapiens |
| MTHFD1L | - |
Homo sapiens |
| NAD-dependent methylenetetrahydrofolate dehydrogenase | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Homo sapiens | the expression of MTHFD1L is significantly affected by the mTORC1-4EBP1-eIF4E axis | down |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | knockdown of MTHFD1L suppresses cell proliferation, colony formation, and tumorigenesis, while it induces apoptosis in oral squamous cell carcinoma (OSCC). Mechanistically, a microarray analysis shows that MTHFD1L suppresses c-MYC and activates p53 signaling by regulating the protein expression of TP53, GADD45A, FAS and JUN. the suppression of MTHFD1L affects c-MYC expression and thereby inhibits tumor cell proliferation and induces apoptosis. MTHFD1L knockdown may affect JUN expression through MAGED and induce cell apoptosis of the tumor. | Homo sapiens |
| physiological function | the NAD-dependent methylenetetrahydrofolate dehydrogenase catalyzes the conversion of 10-formyltetrahydrofolate to formate in embryonic and adult mammalian mitochondria. Methylenetetrahydrofolate dehydrogenase 1-like (MTHFD1L) is a folate cycle enzyme that is involved in the development of various diseases including cancer. Immunohistochemical staining and Kaplan-Meier survival analysis indicate that MTHFD1L upregulation is associated with a poor prognosis in oral squamous cell carcinoma (OSCC). MTHFD1L contributes to the production of NADPH, which is sufficient in combating oxidative stress in OSCC. MTHFD1L directly interacts with the c-MYC proto-oncogene and basic helix-loop-helix (bHLH) transcription factor. MTHFD1L suppresses c-MYC and activates p53 signaling by regulating the protein expression of TP53, GADD45A, FAS and JUN. MTHFD1L directly interacts with the MAGE family member D1 (MAGED). MAGED enhances the phosphorylation of the JUN activator protein 1 (AP-1) transcription factor subunit and activates JUN. Regulation mechanism, overview | Homo sapiens |
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