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Literature summary for 6.3.4.4 extracted from

  • Borza, T.; Iancu, C.V.; Pike, E.; Honzatko, R.B.; Fromm, H.J.
    Variations in the response of mouse isozymes of adenylosuccinate synthetase to inhibitors of physiological relevance (2003), J. Biol. Chem., 278, 6673-6679.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
adenylosuccinate
-
Mus musculus
AMP inhibits the acidic isozyme competitively, weak inhibition of the basic isozyme noncompetitively Mus musculus
D-fructose 1,6-bisphosphate inhibits both isozymes competitively Mus musculus
GDP
-
Mus musculus
GMP
-
Mus musculus
IMP competitive inhibition of the acidic isozyme, noncompetitive of the basic isozyme Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
GTP pH 6.7, 22°C, AdSS1-Tr Mus musculus
0.009
-
IMP pH 6.7, 22°C, AdSS2-Tr Mus musculus
0.012
-
GTP pH 6.7, 22°C, AdSS1 Mus musculus
0.012
-
IMP pH 6.7, 22°C, AdSS2 Mus musculus
0.013
-
GTP pH 6.7, 22°C, AdSS2-Tr Mus musculus
0.015
-
GTP pH 6.7, 22°C, AdSS2 Mus musculus
0.043
-
IMP pH 6.7, 22°C, AdSS1-Tr Mus musculus
0.045
-
IMP pH 6.7, 22°C, AdSS1 Mus musculus
0.14
-
L-aspartate pH 6.7, 22°C, AdSS1 Mus musculus
0.15
-
L-aspartate pH 6.7, 22°C, AdSS1-Tr Mus musculus
0.95
-
L-aspartate pH 6.7, 22°C, AdSS2 Mus musculus
1.03
-
L-aspartate pH 6.7, 22°C, AdSS2-Tr Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ AdSS2 requires 2.0 mM Mg(acetate)2, AdSS1 requires 8.0 mM Mg(acetate)2 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000, SDS-PAGE, recombinant enzyme Mus musculus
86000
-
equilibrium sedimentation ultracentrifugation, AdSS1 Mus musculus
90700
-
equilibrium sedimentation ultracentrifugation, AdSS2 Mus musculus
100700
-
predicted from amino acid sequence, AdSS2 Mus musculus
101100
-
predicted from amino acid sequence, AdSS1 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + IMP + L-aspartate Mus musculus acidic and basic isozymes, which participates in the first committed step of de novo AMP biosynthesis and/or the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
mouse
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + IMP + L-aspartate
-
Mus musculus GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate acidic and basic isozymes, which participates in the first committed step of de novo AMP biosynthesis and/or the purine nucleotide cycle Mus musculus GDP + phosphate + adenylosuccinate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE, recombinant enzyme Mus musculus

Synonyms

Synonyms Comment Organism
Adenylosuccinate synthetase
-
Mus musculus
AdSS1 basic isozyme Mus musculus
AdSS2 acidic isozyme Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.9
-
GTP pH 6.7, 22°C, AdSS1-Tr Mus musculus
4
-
GTP pH 6.7, 22°C, AdSS2-Tr Mus musculus
4.2
-
GTP pH 6.7, 22°C, AdSS2 Mus musculus
5.4
-
GTP pH 6.7, 22°C, AdSS1 Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.012
-
GMP pH 6.7, 22°C, AdSS1 Mus musculus
0.014
-
GMP pH 6.7, 22°C, AdSS2 Mus musculus
0.016
-
D-fructose 1,6-bisphosphate pH 6.7, 22°C, AdSS1, noncompetitive inhibition relative to IMP Mus musculus
0.016
-
adenylosuccinate pH 6.7, 22°C, AdSS2 Mus musculus
0.019
-
GDP pH 6.7, 22°C, AdSS1 Mus musculus
0.019
-
D-fructose 1,6-bisphosphate pH 6.7, 22°C, AdSS2, noncompetitive inhibition relative to IMP Mus musculus
0.021
-
adenylosuccinate pH 6.7, 22°C, AdSS1 Mus musculus
0.03
-
GDP pH 6.7, 22°C, AdSS2 Mus musculus
0.046
-
D-fructose 1,6-bisphosphate pH 6.7, 22°C, AdSS1 Mus musculus
0.059
-
AMP pH 6.7, 22°C, AdSS2 Mus musculus
0.063
-
D-fructose 1,6-bisphosphate pH 6.7, 22°C, AdSS1, noncompetitive inhibition relative to GTP Mus musculus
0.081
-
D-fructose 1,6-bisphosphate pH 6.7, 22°C, AdSS1, noncompetitive inhibition relative to L-aspartate Mus musculus
0.128
-
D-fructose 1,6-bisphosphate pH 6.7, 22°C, AdSS2 Mus musculus
0.4
-
D-fructose 1,6-bisphosphate pH 6.7, 22°C, AdSS2, noncompetitive inhibition relative to GTP Mus musculus
0.67
-
D-fructose 1,6-bisphosphate pH 6.7, 22°C, AdSS2, noncompetitive inhibition relative to L-aspartate Mus musculus
0.7
-
AMP pH 6.7, 22°C, AdSS1 Mus musculus