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Literature summary for 6.3.4.5 extracted from
- Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action (2002), J. Biol. Chem., 277, 15890-15896.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Thermus thermophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| 3-dimensional structure of free enzyme and of complexes with intact ATP, adenylyl imidodiphosphate, arginine or succinate, hanging drop method | Thermus thermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | essential for catalysis | Thermus thermophilus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44815 | - |
4 * 44815, recombinant tAsS, subunit/domain structure, amino acid sequence | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-citrulline + L-aspartate | Thermus thermophilus | second step of urea cycle | AMP + diphosphate + L-argininosuccinate | - |
? | |
| ATP + L-citrulline + L-aspartate | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | second step of urea cycle | AMP + diphosphate + L-argininosuccinate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus thermophilus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate | catalytic mechanism | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-citrulline + L-aspartate | ATP-dependent condensation of citrulline with aspartate, no conformational change upon binding of substrates, mechanism via citrullyl-AMP intermediate, active site structure | Thermus thermophilus | AMP + diphosphate + L-argininosuccinate | - |
r | |
| ATP + L-citrulline + L-aspartate | second step of urea cycle | Thermus thermophilus | AMP + diphosphate + L-argininosuccinate | - |
? | |
| ATP + L-citrulline + L-aspartate | ATP-dependent condensation of citrulline with aspartate, no conformational change upon binding of substrates, mechanism via citrullyl-AMP intermediate, active site structure | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-argininosuccinate | - |
r | |
| ATP + L-citrulline + L-aspartate | second step of urea cycle | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-argininosuccinate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 44815, recombinant tAsS, subunit/domain structure, amino acid sequence | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Argininosuccinate synthetase | - |
Thermus thermophilus |
| ASS | - |
Thermus thermophilus |
| tAsS | - |
Thermus thermophilus |
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Release 2023.1 (January 2023)
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