General Stability | Organism |
---|---|
repeated freezing and thawing accelerates inactivation | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | - |
Saccharomyces cerevisiae | |
azaserine | progressively inhibits glutamine-dependent activity. With NH4+ as amide donor there is an initial 20% inhibition, then the NH4+-dependent activity remains constant | Saccharomyces cerevisiae | |
diisopropyl fluorophosphate | - |
Saccharomyces cerevisiae | |
Mn2+ | can partially replace Mg2+ at 1 mM. At 5 mM inhibition of NAD+ formation | Saccharomyces cerevisiae | |
p-chloromercuribenzoate | glutamine-dependent and NH4+-dependent activity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
ATP | pH 7.6 | Saccharomyces cerevisiae | |
0.19 | - |
deamido-NAD+ | pH 7.6 | Saccharomyces cerevisiae | |
5 | - |
Gln | pH 7.6 | Saccharomyces cerevisiae | |
6.4 | - |
NH4+ | pH 8.6 | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Mg2+, at 5 mM 30% of the activity relative to Mg2+ | Saccharomyces cerevisiae | |
K+ | required | Saccharomyces cerevisiae | |
Mg2+ | required | Saccharomyces cerevisiae | |
Mn2+ | can partially replace Mg2+ at 1 mM. At 5 mM inhibition of NAD+ formation | Saccharomyces cerevisiae | |
NH4+ | as effective as K+ in stimulating activity | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
630000 | - |
high speed equilibrium ultracentrifugation | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
4°C, 0.2 mg per ml, 10 d, 60% loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + Gln + hydroxylamine | - |
Saccharomyces cerevisiae | AMP + diphosphate + hydroxamate analog + ? | - |
? | |
ATP + deamido-NAD+ + L-Gln | - |
Saccharomyces cerevisiae | AMP + diphosphate + NAD+ + L-Glu | - |
? | |
ATP + deamido-NAD+ + NH4+ | - |
Saccharomyces cerevisiae | AMP + diphosphate + NAD+ | - |
? | |
Gln + hydroxylamine | - |
Saccharomyces cerevisiae | AMP + diphosphate + hydroxamate analog + ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover number: 1260, NAD+ production | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.4 | broad peak of activity with a maximum around 7.6, with Gln as the amide donor | Saccharomyces cerevisiae |
8.4 | 8.8 | with NH4+ as amide donor | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.8 | 9.3 | 7.8: about 40% of maximal activity, 9.3: about 50% of maximal activity, with NH4+ as amide donor | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | overnight, no loss of activity | Saccharomyces cerevisiae |