Cloned (Comment) | Organism |
---|---|
expression of His-tagged NAD+ synthase in Escherichia coli | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
x * 98000, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + L-glutamine + H2O | Mycobacterium tuberculosis | last step in NAD+ biosynthesis | AMP + diphosphate + NAD+ + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
NAD+ synthase is encoded by the MTCY428.08 gene | - |
Purification (Comment) | Organism |
---|---|
recombinant NAD+ synthase, Ni2+ chelate resin affinity chromatography | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.09 | - |
glutamine-dependent activity | Mycobacterium tuberculosis |
0.23 | - |
NH3 dependent activity | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + L-glutamine + H2O | - |
Mycobacterium tuberculosis | AMP + diphosphate + NAD+ + L-glutamate | - |
? | |
ATP + deamido-NAD+ + L-glutamine + H2O | last step in NAD+ biosynthesis | Mycobacterium tuberculosis | AMP + diphosphate + NAD+ + L-glutamate | - |
? | |
ATP + deamido-NAD+ + NH3 + H2O | NAD+ synthase is able to use ammonia or glutamine as nitrogen source | Mycobacterium tuberculosis | AMP + diphosphate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 98000, SDS-PAGE | Mycobacterium tuberculosis |