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Literature summary for 6.3.5.13 extracted from

  • Muench, D.; Roemer, T.; Lee, S.H.; Engeser, M.; Sahl, H.G.; Schneider, T.
    Identification and in vitro analysis of the GatD/MurT enzyme-complex catalyzing lipid II amidation in Staphylococcus aureus (2012), PLoS Pathog., 8, e1002509 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.5.13

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
C94G mutation in subunit GaTD, abolishes lipid II amidation Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol Staphylococcus aureus
-
 ADP + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol
-
 ?
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol Staphylococcus aureus N315
-
 ADP + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol
-
 ?
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O Staphylococcus aureus
-
 ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate
-
 ?
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O Staphylococcus aureus N315
-
 ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate
-
 ?
beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + NH3 Staphylococcus aureus
-
 beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + phosphate
-
 ?
beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + NH3 Staphylococcus aureus N315
-
 beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + phosphate
-
 ?
L-glutamine + H2O Staphylococcus aureus
-
 L-glutamate + NH3
-
 ?
L-glutamine + H2O Staphylococcus aureus N315
-
 L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus A0A0H3JN63 and A0A0H3JUU7 A0A0H3JN63 i.e subunit GatD, A0A0H3JUU7 i.e. subunit MurT
-
Staphylococcus aureus N315 A0A0H3JN63 and A0A0H3JUU7 A0A0H3JN63 i.e subunit GatD, A0A0H3JUU7 i.e. subunit MurT
-

Purification (Commentary)

Purification (Comment) Organism
recombinant MurT/GatD complex Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol
-
 Staphylococcus aureus ADP + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol
-
 ?
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol
-
 Staphylococcus aureus N315 ADP + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol
-
 ?
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O
-
 Staphylococcus aureus ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate
-
 ?
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O
-
 Staphylococcus aureus N315 ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate
-
 ?
ATP + glutamate in lipid I + L-glutamine + H2O
-
 Staphylococcus aureus ADP + phosphate + glutamine in lipid I + L-glutamate
-
 ?
ATP + glutamate in lipid II + L-glutamine + H2O
-
 Staphylococcus aureus ADP + phosphate + glutamine in lipid II + L-glutamate
-
 ?
ATP + glutamate in lipid II-Gly5 + L-glutamine + H2O
-
 Staphylococcus aureus ADP + phosphate + glutamine in lipid II-Gly5 + L-glutamate
-
 ?
beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + NH3
-
 Staphylococcus aureus beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + phosphate
-
 ?
beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + NH3
-
 Staphylococcus aureus N315 beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + phosphate
-
 ?
L-glutamine + H2O
-
 Staphylococcus aureus L-glutamate + NH3
-
 ?
L-glutamine + H2O
-
 Staphylococcus aureus N315 L-glutamate + NH3
-
 ?
additional information both MurT and GatD are required for the reaction Staphylococcus aureus ?
-
 ?
additional information both MurT and GatD are required for the reaction Staphylococcus aureus N315 ?
-
 ?

Synonyms

Synonyms Comment Organism
GatD/MurT
-
 Staphylococcus aureus
SA1707 subunit Gatd Staphylococcus aureus
SA1708 subunit Murt Staphylococcus aureus

General Information

General Information Comment Organism
physiological function enzyme complex catalyzes the amidation of the peptidoglycan complex, which is important for polymerization of cell wall building blocks. Inactivation of the GatD active site abolishes lipid II amidation Staphylococcus aureus