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Literature summary for 6.3.5.3 extracted from

  • Buchanan, J.M.; Ohnoki, S.; Hong, B.S.
    2-Formamido-N-ribosylacetamide 5'-phosphate:L-glutamine amido-ligase (adenosine diphosphate) (1978), Methods Enzymol., 51, 193-201.
    View publication on PubMed
Search for more literature for 6.3.5.3

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxo-L-norleucine competitive analogue of Gln binds irreversibly at the Gln active site Gallus gallus
albizziin competitive analogue of Gln binds irreversibly at the Gln active site Gallus gallus
Cyanate
-
 Gallus gallus
hydroxylamine
-
 Gallus gallus
iodoacetamide
-
 Gallus gallus
iodoacetate
-
 Gallus gallus
O-diazoacetyl-L-Ser i.e. L-azaserine, competitive analogue of Gln binds irreversibly at the Gln active site Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 5'-phosphoribosylformylglycinamide
-
 Gallus gallus
0.2
-
 Gln
-
 Gallus gallus
0.4
-
 ATP
-
 Gallus gallus
80 100 NH4+
-
 Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
133000 135000
-
 Salmonella enterica subsp. enterica serovar Typhimurium
133000 135000
-
 Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Reaction

Reaction Comment Organism Reaction ID
ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate Gln adds first to the enzyme with ATP and 5'-phosphoribosylformylglycinamide adding randomly subsequently Gallus gallus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu
-
 ?
ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O
-
 Gallus gallus ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu
-
 ?
ATP + 5'-phosphoribosylformylglycinamide + NH4+ + H2O
-
 Gallus gallus ADP + phosphate + 5'-phosphoribosylformylglycinamidine
-
 ?
ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O ATP in form of MgATP2- Gallus gallus ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate
-
 ?
additional information glutamyl derivatives, e.g. gamma-glutamylhydroxamate, gamma-glutamyl hydrazine, gamma-glutamyl esters, and gamma-glutamyl thioesters are hydrolyzed with strict dependence on Mg2+, ATP, and 5'-phosphoribosylformylglycinamide Gallus gallus ?
-
 ?