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Literature summary for 6.3.5.4 extracted from
- Glutamine-dependent asparagine synthetase from leukemia cells. Chloride dependence mechanism of action and inhibition (1972), J. Biol. Chem., 247, 6708-6719.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-dicarboxypyridine | - |
Mus musculus |  |
| 2,4-Dicarboxypyridine | - |
Mus musculus | |
| 2,5-Dicarboxypyridine | - |
Mus musculus | |
| 2,6-dicarboxypyridine | - |
Mus musculus | |
| 2-carboxypyridine | - |
Mus musculus | |
| 3,4-Dicarboxypyridine | - |
Mus musculus | |
| 3,5-Dicarboxypyridine | - |
Mus musculus | |
| 4-Carboxypyridine | - |
Mus musculus | |
| Albizzine | - |
Mus musculus | |
| alpha,beta-methylene ATP | - |
Mus musculus | |
| aminomalonic acid | - |
Mus musculus | |
| ammonium maleamate | weak | Mus musculus | |
| AMP | - |
Mus musculus | |
| Arg | - |
Mus musculus | |
| Asn | - |
Mus musculus | |
| beta,gamma-methylene ATP | - |
Mus musculus | |
| D-Aspartic acid | weak | Mus musculus | |
| diphosphate | - |
Mus musculus | |
| DL-alpha-Aminotricarballylic acid | weak | Mus musculus | |
| Glu | - |
Mus musculus | |
| Gly-L-Asn | weak | Mus musculus | |
| L-2-Amino-4-oxo-5-chloropentanoic acid | - |
Mus musculus | |
| L-2-Amino-4-oxo-5-hydroxypentanoic acid | - |
Mus musculus | |
| L-beta-Aspartate ethyl ester | - |
Mus musculus | |
| L-Malic acid | weak | Mus musculus | |
| N-Benzyloxycarbonyl-L-Asn | weak | Mus musculus | |
| N-Benzyloxycarbonyl-L-aspartate | weak | Mus musculus | |
| N-Methyl-DL-aspartic acid | - |
Mus musculus | |
| Phthalic acid | weak | Mus musculus | |
| S-Carbamoylcysteine | - |
Mus musculus | |
| S-methyl-L-cysteine | - |
Mus musculus | |
| S-methyl-L-cysteine-(RS)-sulfoximine | weak | Mus musculus | |
| Trp | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
ATP | - |
Mus musculus | |
| 0.9 | - |
L-Asp | - |
Mus musculus | |
| 1 | - |
L-Gln | - |
Mus musculus | |
| 9 | - |
NH4+ | - |
Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Br- | can partially replace Cl- in activation | Mus musculus | |
| Cl- | required for glutaminase and glutamine-dependent synthetase activity, not for the NH4+-dependent activity | Mus musculus | |
| I- | can partially replace Cl- in activation | Mus musculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leukemia cell line | Asn-resistant leukemia cells | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Asp + L-Gln | - |
Mus musculus | AMP + diphosphate + Asn + Glu | - |
? | |
| ATP + L-Asp + NH3 | - |
Mus musculus | AMP + diphosphate + Asn | - |
? | |
| additional information | enzyme has inherent glutaminase activity | Mus musculus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8.2 | - |
Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 8.8 | minor variation of activity in this pH range | Mus musculus |
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