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Literature summary for 6.4.1.1 extracted from

  • Charles, A.M.; Willer, D.W.
    Pyruvate carboxylase from Thiobacillus novellus: properties and possible function (1984), Can. J. Microbiol., 30, 532-539.
No PubMed abstract available
Search for more literature for 6.4.1.1

Activating Compound

Activating Compound Comment Organism Structure
acetoacetyl-CoA activation in decreasing order: acetyl-CoA, n-propanyl-CoA, n-butanoyl-CoA, malonyl-CoA/ CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella
acetyl-CoA best activator Starkeya novella
acetyl-CoA activation in decreasing order: acetyl-CoA, n-propanoyl-CoA, n-butanoyl-CoA, malonyl-CoA, CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella
acyl-CoA derivatives activation in decreasing order: acetyl-CoA, n-propanyl-CoA, n-butanoyl-CoA, malonyl-CoA/ CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella
malonyl-CoA activation in decreasing order: acetyl-CoA, n-propanyl-CoA, n-butanoyl-CoA, malonyl-CoA/ CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella
n-butanoyl-CoA activation in decreasing order: acetyl-CoA, n-propanyl-CoA, n-butanoyl-CoA, malonyl-CoA/ CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella
n-propanoyl-CoA activation in decreasing order: acetyl-CoA, n-propanyl-CoA, n-butanoyl-CoA, malonyl-CoA/ CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella
oleoyl-CoA activation in decreasing order: acetyl-CoA, n-propanyl-CoA, n-butanoyl-CoA, malonyl-CoA/ CoA, acetoacetyl-CoA, oleoyl-CoA Starkeya novella

General Stability

General Stability Organism
freezing and thawing over a 16 h period results in rapid loss of activity Starkeya novella

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Starkeya novella
CTP in presence of ATP Starkeya novella
GTP in presence of ATP Starkeya novella
HgCl2 addition of Cys results in restoration of significant activity Starkeya novella
Hydroxypyruvate
-
 Starkeya novella
ITP in presence of ATP Starkeya novella
Mercuric acetate addition of Cys results in restoration of significant activity Starkeya novella
oxaloacetate
-
 Starkeya novella
p-hydroxymercuribenzoate
-
 Starkeya novella
phosphate
-
 Starkeya novella
Tris
-
 Starkeya novella
UTP in presence of ATP Starkeya novella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 ATP
-
 Starkeya novella
0.25
-
 pyruvate
-
 Starkeya novella
0.27
-
 HCO3-
-
 Starkeya novella

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required, activates with 58% of the efficiency relative to Mg2+ Starkeya novella
Cs+ monovalent cation required, order of efficiency: Rb+, K+, Cs+/ NH4+ Starkeya novella
K+ monovalent cation required, order of efficiency: Rb+, K+, Cs+/ NH4+ Starkeya novella
Mg2+ divalent cation required, Mg2+ is most effective, Km: 0.44 mM Starkeya novella
Mn2+ divalent cation required, activates with 66% of the efficiency relative to Mg2+ Starkeya novella
NH4+ monovalent cation required, order of efficiency: Rb+, K+, Cs+/ NH4+ Starkeya novella
Rb+ monovalent cation required, Rb+ is most effective Starkeya novella

Organism

Organism UniProt Comment Textmining
Starkeya novella
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Starkeya novella

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.03
-
-
 Starkeya novella

Storage Stability

Storage Stability Organism
-20°C, pH 7.8, 2 weeks, 40% loss of activity Starkeya novella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + HCO3-
-
 Starkeya novella ADP + phosphate + oxaloacetate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 35
-
 Starkeya novella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Starkeya novella