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Literature summary for 6.4.1.1 extracted from

  • Branson, J.P.; Nezic, M.; Jitrapakdee, S.; Wallace, J.C.; Attwood, P.V.
    Kinetic characterization of yeast pyruvate carboxylase isozyme Pyc1 and the Pyc1 mutant, C249A (2004), Biochemistry, 43, 1075-1081.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information acetyl-CoA and K+ have no effect on ADP phosphorylation by carbamoyl phosphate Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
Pyc1 isoform and C249A mutant Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C249A only small effects on enzyme activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
ATP pH 7.8, 30°C, C249A mutant Saccharomyces cerevisiae
0.07
-
ATP pH 7.8, 30°C, wild-type Pyc1 Saccharomyces cerevisiae
0.45
-
pyruvate pH 7.8, 30°C, C249A mutant Saccharomyces cerevisiae
0.5
-
pyruvate pH 7.8, 30°C, wild-type Pyc1 Saccharomyces cerevisiae
1.36
-
HCO3- pH 7.8, 30°C, wild-type Pyc1 Saccharomyces cerevisiae
1.7
-
Carbamoyl phosphate pH 7.8, 30°C, C249A mutant, with acetyl-CoA and K+ Saccharomyces cerevisiae
2.3
-
HCO3- pH 7.8, 30°C, C249A mutant Saccharomyces cerevisiae
10.7
-
Carbamoyl phosphate pH 7.8, 30°C, C249A mutant, without acetyl-CoA and K+ Saccharomyces cerevisiae
11.3
-
Carbamoyl phosphate pH 7.8, 30°C, wild-type Pyc1, with acetyl-CoA and K+ Saccharomyces cerevisiae
12.3
-
Carbamoyl phosphate pH 7.8, 30°C, wild-type Pyc1, without acetyl-CoA and K+ Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate + HCO3- Saccharomyces cerevisiae
-
ADP + phosphate + oxaloacetate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Pyc1 isoform and C249A mutant Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + carbamoyl phosphate
-
Saccharomyces cerevisiae ATP + ?
-
?
ATP + pyruvate + HCO3-
-
Saccharomyces cerevisiae ADP + phosphate + oxaloacetate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.043
-
Carbamoyl phosphate pH 7.8, 30°C, C249A mutant, with acetyl-CoA and K+ Saccharomyces cerevisiae
0.11
-
Carbamoyl phosphate pH 7.8, 30°C, wild-type Pyc1, with acetyl-CoA and K+ Saccharomyces cerevisiae
0.11
-
Carbamoyl phosphate pH 7.8, 30°C, wild-type Pyc1, without acetyl-CoA and K+ Saccharomyces cerevisiae
0.13
-
Carbamoyl phosphate pH 7.8, 30°C, C249A mutant, without acetyl-CoA and K+ Saccharomyces cerevisiae