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Literature summary for 6.4.1.1 extracted from

  • Jitrapakdee, S.; Adina-Zada, A.; Besant, P.G.; Surinya, K.H.; Cleland, W.W.; Wallace, J.C.; Attwood, P.V.
    Differential regulation of the yeast isozymes of pyruvate carboxylase and the locus of action of acetyl CoA (2007), Int. J. Biochem. Cell Biol., 39, 1211-1223.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information construction of chimeric enzymes of Pyc1 and Pyc2 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
ATP 30°C, pH 7.8 Saccharomyces cerevisiae
0.5
-
pyruvate 30°C, pH 7.8 Saccharomyces cerevisiae
1.36
-
HCO3- 30°C, pH 7.8 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate + HCO3- Saccharomyces cerevisiae Pyc1 and Pyc2 display different allosteric properties with respect to acetyl CoA activation and aspartate inhibition, with Pyc1 showing a higher degree of cooperativity than Pyc2, even in the absence of aspartate ADP + phosphate + oxaloacetate
-
?
ATP + pyruvate + HCO3- Saccharomyces cerevisiae DM18 Pyc1 and Pyc2 display different allosteric properties with respect to acetyl CoA activation and aspartate inhibition, with Pyc1 showing a higher degree of cooperativity than Pyc2, even in the absence of aspartate ADP + phosphate + oxaloacetate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P11154 strain DM18
-
Saccharomyces cerevisiae P32327
-
-
Saccharomyces cerevisiae DM18 P32327
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + HCO3- Pyc1 and Pyc2 display different allosteric properties with respect to acetyl CoA activation and aspartate inhibition, with Pyc1 showing a higher degree of cooperativity than Pyc2, even in the absence of aspartate Saccharomyces cerevisiae ADP + phosphate + oxaloacetate
-
?
ATP + pyruvate + HCO3- Pyc1 and Pyc2 display different allosteric properties with respect to acetyl CoA activation and aspartate inhibition, with Pyc1 showing a higher degree of cooperativity than Pyc2, even in the absence of aspartate Saccharomyces cerevisiae DM18 ADP + phosphate + oxaloacetate
-
?

Synonyms

Synonyms Comment Organism
Pyc1
-
Saccharomyces cerevisiae
PYC2
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60
-
ATP 30°C, pH 7.8 Saccharomyces cerevisiae
60
-
pyruvate 30°C, pH 7.8 Saccharomyces cerevisiae
60
-
HCO3- 30°C, pH 7.8 Saccharomyces cerevisiae