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Literature summary for 6.4.1.2 extracted from

  • Charles, D.J.; Cherry, J.H.
    Purification and characterization of acetyl-CoA carboxylase from developing soybean seeds (1986), Phytochemistry, 25, 1067-1071.
No PubMed abstract available
Search for more literature for 6.4.1.2

General Stability

General Stability Organism
DTT, glycerol, Triton X-100, and high concentrations of the enzyme are required for stability Glycine max

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Glycine max
AMP
-
 Glycine max
citrate 10 mM Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 ATP 9686 enzyme Glycine max
0.17
-
 acetyl-CoA 9686 enzyme Glycine max
0.32
-
 acetyl-CoA Wayne enzyme Glycine max
0.46
-
 ATP Wayne enzyme Glycine max
1.13
-
 HCO3- Wayne enzyme Glycine max
1.56
-
 HCO3- 9686 enzyme Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, 9686 enzyme, SDS-PAGE Glycine max
58000
-
 x * 58000 + x * 65000 + x * 240000, SDS-PAGE Glycine max
65000
-
 x * 58000 + x * 65000 + x * 240000, SDS-PAGE Glycine max
240000
-
 x * 58000 + x * 65000 + x * 240000, SDS-PAGE Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
 2 lines of seeds: 9686 and Wayne
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seed 2 lines of seeds: 9686 and Wayne Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA + HCO3- absolute specificity for acetyl-CoA Glycine max ADP + phosphate + malonyl-CoA
-
 ?
ATP + butyryl-CoA + HCO3- no activity Glycine max ADP + ethylmalonyl-CoA + phosphate
-
 ?
ATP + propionyl-CoA + HCO3- no activity Glycine max ADP + phosphate + methylmalonyl-CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 58000 + x * 65000 + x * 240000, SDS-PAGE Glycine max
? x * 40000, 9686 enzyme, SDS-PAGE Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 9686 enzyme Glycine max
8.2
-
 Wayne enzyme Glycine max

Cofactor

Cofactor Comment Organism Structure
biotin covalent attachment of the biotin prosthetic group Glycine max