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Literature summary for 6.4.1.2 extracted from

  • Mishina, M.; Kamiryo, T.; Numa, S.
    Acetyl-CoA carboxylase from Candida lipolytica. EC 6.4.1.2 Acetyl-CoA:carbon-dioxide ligase (ADP-forming) (1981), Methods Enzymol., 71, 37-44.
No PubMed abstract available
Search for more literature for 6.4.1.2

Activating Compound

Activating Compound Comment Organism Structure
citrate no effect Yarrowia lipolytica
Polyethylene glycols of MW 7500 or 3000, activate Yarrowia lipolytica

General Stability

General Stability Organism
extremely labile without stabilizer, e.g. glycerol Yarrowia lipolytica

Inhibitors

Inhibitors Comment Organism Structure
acetone
-
 Yarrowia lipolytica
dioxane
-
 Yarrowia lipolytica
ethanol
-
 Yarrowia lipolytica
ethylene glycol monomethyl ether
-
 Yarrowia lipolytica
glycerol
-
 Yarrowia lipolytica
tetrahydrofuran
-
 Yarrowia lipolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 ATP ATP Yarrowia lipolytica
0.26
-
 acetyl-CoA
-
 Yarrowia lipolytica
3.7
-
 HCO3-
-
 Yarrowia lipolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
230000
-
 x * 230000, SDS-PAGE Yarrowia lipolytica

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Yarrowia lipolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Yarrowia lipolytica

Storage Stability

Storage Stability Organism
-70°C, 20% glycerol, stable for at least 6 months Yarrowia lipolytica
0°C, 20% glycerol, 24 h, stable Yarrowia lipolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA + HCO3-
-
 Yarrowia lipolytica ADP + phosphate + malonyl-CoA
-
 ?
ATP + acetyl-CoA + hydrogencarbonate ATP in form of MgATP2- Yarrowia lipolytica ADP + phosphate + malonyl-CoA
-
 ?
ATP + propionyl-CoA + HCO3- 13% of the activity relative to acetyl-CoA Yarrowia lipolytica ADP + phosphate + methylmalonyl-CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 230000, SDS-PAGE Yarrowia lipolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9 8.1
-
 Yarrowia lipolytica

Cofactor

Cofactor Comment Organism Structure
biotin contains 1 mol of biotin per 263000 g of protein Yarrowia lipolytica