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Literature summary for 6.4.1.2 extracted from
- Characterization of acetyl-CoA/propionyl-CoA carboxylase in Metallosphaera sedula. Carboxylating enzyme in the 3-hydroxypropionate cycle for autotrophic carbon fixation (2003), Eur. J. Biochem., 270, 736-744.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| each subunit individually cloned, carboxyl transferase expressed in Escherichia coli | Metallosphaera sedula |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Metallosphaera sedula |  |
| Avidin | reversible by addition of biotin | Metallosphaera sedula | |
| CoA | - |
Metallosphaera sedula | |
| malonyl-CoA | - |
Metallosphaera sedula | |
| succinyl-CoA | - |
Metallosphaera sedula | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
ATP | pH 7.5, 65°C | Metallosphaera sedula | |
| 0.06 | - |
acetyl-CoA | pH 7.5, 65°C | Metallosphaera sedula | |
| 0.3 | - |
HCO3- | pH 7.5, 65°C | Metallosphaera sedula | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially replace for Mg2+ | Metallosphaera sedula | |
| Co2+ | can partially replace for Mg2+ | Metallosphaera sedula | |
| Mg2+ | required to form a complex with ATP | Metallosphaera sedula | |
| Mn2+ | can partially replace for Mg2+ | Metallosphaera sedula | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 560000 | - |
gel filtration | Metallosphaera sedula |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetyl-CoA + HCO3- | Chloroflexus aurantiacus | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | Sulfuracidifex metallicus | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | Metallosphaera sedula | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | Chloroflexus aurantiacus OK-70-fl / DSM 636 | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chloroflexus aurantiacus | - |
- |
- |
| Chloroflexus aurantiacus OK-70-fl / DSM 636 | - |
- |
- |
| Metallosphaera sedula | Q8J2Z4 | - |
- |
| Metallosphaera sedula | Q8J2Z5 | - |
- |
| Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | Q8J2Z4 | - |
- |
| Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | Q8J2Z5 | - |
- |
| Sulfuracidifex metallicus | - |
DSM 6482 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Metallosphaera sedula |
| partial | Chloroflexus aurantiacus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.2 | - |
purified enzyme, pH 7.5, 65°C | Metallosphaera sedula |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetyl-CoA + HCO3- | - |
Chloroflexus aurantiacus | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | - |
Metallosphaera sedula | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | - |
Sulfuracidifex metallicus | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Chloroflexus aurantiacus | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Sulfuracidifex metallicus | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Metallosphaera sedula | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | - |
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | - |
Chloroflexus aurantiacus OK-70-fl / DSM 636 | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Chloroflexus aurantiacus OK-70-fl / DSM 636 | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + propionyl-CoA + HCO3- | second activity of the protein representing EC 6.4.1.3 | Metallosphaera sedula | ADP + phosphate + methylmalonyl-CoA | - |
? | |
| ATP + propionyl-CoA + HCO3- | second activity of the protein representing EC 6.4.1.3 | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + methylmalonyl-CoA | - |
? | |
| GTP + acetyl-CoA + HCO3- | 10% of the activity obtained with ATP | Metallosphaera sedula | GDP + phosphate + malonyl-CoA | - |
? | |
| GTP + acetyl-CoA + HCO3- | 10% of the activity obtained with ATP | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | GDP + phosphate + malonyl-CoA | - |
? | |
| UTP + acetyl-CoA + HCO3- | 40% of the activity obtained with ATP | Metallosphaera sedula | UDP + phosphate + malonyl-CoA | - |
? | |
| UTP + acetyl-CoA + HCO3- | 40% of the activity obtained with ATP | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | UDP + phosphate + malonyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit) | Chloroflexus aurantiacus |
| multimer | most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit) | Sulfuracidifex metallicus |
| multimer | most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit) | Metallosphaera sedula |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetyl-CoA/propionyl-CoA carboxylase | enzyme acts on both substrates | Chloroflexus aurantiacus |
| acetyl-CoA/propionyl-CoA carboxylase | enzyme acts on both substrates | Sulfuracidifex metallicus |
| acetyl-CoA/propionyl-CoA carboxylase | enzyme acts on both substrates | Metallosphaera sedula |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Metallosphaera sedula |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 85 | - |
Metallosphaera sedula |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | 8 | acetyl-CoA | pH 7.5, 65°C | Metallosphaera sedula | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Metallosphaera sedula |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Metallosphaera sedula |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | enzyme-bound | Chloroflexus aurantiacus | |
| biotin | enzyme-bound | Sulfuracidifex metallicus | |
| biotin | enzyme-bound | Metallosphaera sedula | |
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