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Literature summary for 6.4.1.2 extracted from

  • Benson, B.K.; Meades, G.J.; Grove, A.; Waldrop, G.L.
    DNA inhibits catalysis by the carboxyltransferase subunit of acetyl-CoA carboxylase: implications for active site communication (2008), Protein Sci., 17, 34-42.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.4.1.2

Inhibitors

Inhibitors Comment Organism Structure
coenzyme A-carboxybiotin coenzyme A is covalently attached to carboxybiotin, bisubstrate inhibitor Escherichia coli
Double-stranded DNA inhibits catalysis by the carboxyltransferase subunit of acetyl-CoA carboxylase by complex formation and influencing the active site structure, while substrates malonyl-CoA and biocytin inhibit DNA-carboxyltransferase complex formation, overview. DNA and heparin can also bind to the enzyme simultaneously, but the binding of either molecule has a strong synergistic effect on binding of the other, binding structure and inhibition mechanism, overview Escherichia coli
heparin the DNA mimic inhibits the reaction catalyzed by carboxyltransferase in a competitive manner with respect to both malonyl-CoA and biocytin. DNA and heparin can also bind to the enzyme simultaneously, but the binding of either molecule has a strong synergistic effect on binding of the other, binding structure and inhibition mechanism, overview Escherichia coli
single-stranded DNA inhibits the reaction catalyzed by carboxyltransferase in a competitive manner with respect to both malonyl-CoA and biocytin. DNA and heparin can also bind to the enzyme simultaneously, but the binding of either molecule has a strong synergistic effect on binding of the other, binding structure and inhibition mechanism, overview Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli
additional information the zinc domain in bacterial carboxyltransferase belongs to the zinc ribbon class of zinc fingers, Zn domains are commonly associated with DNA binding Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetyl-CoA + HCO3- Escherichia coli ACC catalyzes the first committed step in the synthesis of long-chain fatty acids ADP + phosphate + malonyl-CoA
-
 ?
additional information Escherichia coli acetyl-CoA carboxylase is a multifunctional biotin-dependent enzyme that catalyzes the first committed and regulated step in fatty acid biosynthesis in bacteria via a two-step reaction. Biotin carboxylase, EC 6.3.4.14, catalyzes the first half-reaction, which is an ATP-dependent carboxylation of biotin to form carboxybiotin. The second half-reaction is catalyzed by carboxyltransferase, EC 6.4.1.2, which transfers the carboxyl group from carboxybiotin to acetyl-CoA to generate malonyl-CoA ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA + HCO3-
-
 Escherichia coli ADP + phosphate + malonyl-CoA
-
 ?
ATP + acetyl-CoA + HCO3- ACC catalyzes the first committed step in the synthesis of long-chain fatty acids Escherichia coli ADP + phosphate + malonyl-CoA
-
 ?
additional information acetyl-CoA carboxylase is a multifunctional biotin-dependent enzyme that catalyzes the first committed and regulated step in fatty acid biosynthesis in bacteria via a two-step reaction. Biotin carboxylase, EC 6.3.4.14, catalyzes the first half-reaction, which is an ATP-dependent carboxylation of biotin to form carboxybiotin. The second half-reaction is catalyzed by carboxyltransferase, EC 6.4.1.2, which transfers the carboxyl group from carboxybiotin to acetyl-CoA to generate malonyl-CoA Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
ACC
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli
biotin dependent on Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0012
-
 heparin versus malonyl-CoA Escherichia coli
0.0022
-
 heparin versus biocytin Escherichia coli
0.0342
-
 single-stranded DNA versus biocytin Escherichia coli
0.085
-
 single-stranded DNA versus malonyl-CoA Escherichia coli