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Literature summary for 6.4.1.4 extracted from
- 3-Methylcrotonyl-CoA and geranyl-CoA carboxylases from Pseudomonas citronellolis (1981), Methods Enzymol., 71, 791-799.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
3-methylcrotonoyl-CoA | - |
Pseudomonas citronellolis |  |
| 0.138 | - |
(E)-crotonoyl-CoA | - |
Pseudomonas citronellolis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B | Pseudomonas citronellolis |
| 73000 | - |
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B | Pseudomonas citronellolis |
| 520000 | 580000 | gel filtration | Pseudomonas citronellolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas citronellolis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas citronellolis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.54 | - |
- |
Pseudomonas citronellolis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, approximately 25% loss of activity per month | Pseudomonas citronellolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Pseudomonas citronellolis | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
| ATP + crotonoyl-CoA + HCO3- | (E)-crotonoyl-CoA | Pseudomonas citronellolis | ? | - |
? | |
| ATP + crotonoyl-CoA + HCO3- | 44% of the activity relative to 3-methylcrotonoyl-CoA | Pseudomonas citronellolis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B | Pseudomonas citronellolis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | enzyme contains biotin | Pseudomonas citronellolis | |
| biotin | biotin is bound to the heavier subunit | Pseudomonas citronellolis | |
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Release 2023.1 (January 2023)
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