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Literature summary for 6.5.1.1 extracted from
- Eukaryotic DNA ligase. Purification and properties of the enzyme from bovine thymus and immunochemical studies of the enzyme from animal tissues (1982), J. Biol. Chem., 257, 4758-4763.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| polyamines | polyamines activate at suboptimal concentrations of Mg2+ | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Bos taurus |  |
| Mg2+ | Km: 0.9 mM | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | 130000 | sucrose density gradient centrifugation, highly asymetric shape | Bos taurus |
| 130000 | - |
1 * 130000, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for at least 2 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Bos taurus | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 1 * 130000, SDS-PAGE | Bos taurus |
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