Literature summary for 6.5.1.1 extracted from

Brown, G.W.; Ray, D.S.
Purification and characterization of DNA ligase I from the trypanosomatid Crithidia fasciculata (1992), Nucleic Acids Res., 20, 3905-3910.

Search for more literature for 6.5.1.1

Inhibitors

Inhibitors	Comment	Organism	Structure
K+	50 mM, 50% inhibition	Crithidia fasciculata
NEM	-	Crithidia fasciculata

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00034	-	ATP	-	Crithidia fasciculata

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Crithidia fasciculata

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	gel filtration, glycerol density gradient sedimentation	Crithidia fasciculata

Organism

Organism	UniProt	Comment	Textmining
Crithidia fasciculata	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DNA ligase I	Crithidia fasciculata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Crithidia fasciculata

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	joins oligo(rA) annealed to poly(dT) with 250fold lower efficiency than it joins oligo(dT) annealed to poly(dA), ligates blunt-ended DNA fragments in the presence of 15% polyethylene glycol 80000	Crithidia fasciculata	AMP + diphosphate + (deoxyribonucleotide)n+m	-	?

Subunits

Subunits	Comment	Organism
monomer	x * 80000, SDS-PAGE	Crithidia fasciculata

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
50	-	K+	-	Crithidia fasciculata

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Release 2023.1 (January 2023)