Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, polyhistidine-tagged enzyme | Enterobacteria phage T3 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 2 mM 50% inhibition | Enterobacteria phage T3 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0144 | - |
ATP | ligation of cohesive DNA | Enterobacteria phage T3 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, increases activity 9fold | Enterobacteria phage T3 | |
Mg2+ | 5 mM, increases activity 9fold | Enterobacteria phage T3 | |
Mn2+ | 5 mM, increases activity 7fold | Enterobacteria phage T3 | |
NaCl | promotes enzyme activity, optimal concentration: 300 mM | Enterobacteria phage T3 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Enterobacteria phage T3 | - |
AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Enterobacteria phage T3 | - |
dAMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacteria phage T3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant polyhistidine-tagged enzyme | Enterobacteria phage T3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Enterobacteria phage T3 | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Enterobacteria phage T3 | dAMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
8 | - |
- |
Enterobacteria phage T3 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Enterobacteria phage T3 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | pH 6.5: about 80% of maximal activity, pH 8.5: about 85% of maximal activity | Enterobacteria phage T3 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Enterobacteria phage T3 | calculation from amino acid sequence | - |
5.14 |