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Literature summary for 6.5.1.1 extracted from

  • Guenther Sillero, M.A.; de Diego, A.; Perez-Zuniga, F.J.; Sillero, A.
    Synthesis of bisphosphonate derivatives of ATP by T4 DNA ligase, ubiquitin activating enzyme (E1) and other ligases (2008), Biochem. Pharmacol., 75, 1959-1965.
    View publication on PubMed
Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Tequatrovirus T4

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by pamidronate Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 methylenebisphosphonate in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C Tequatrovirus T4
0.08
-
 clodronate in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C Tequatrovirus T4
0.73
-
 etidronate in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C Tequatrovirus T4
1.3
-
 tripolyphosphate in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m T4 DNA ligase catalyzes the formation of phosphodiester bonds between neighboring 3'-hydroxyl and 5'-phosphate ends in doublestranded DNA Tequatrovirus T4 AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
ATP + clodronate
-
 Tequatrovirus T4 adenosine 5'-triphosphate derivative of clodronate + diphosphate
-
 ?
ATP + etidronate
-
 Tequatrovirus T4 adenosine 5'-triphosphate derivative of etidronate + diphosphate
-
 ?
ATP + methylenebisphosphonate
-
 Tequatrovirus T4 adenosine 5'-triphosphate derivative of methylenebisphosphonate + diphosphate
-
 ?
ATP + tripolyphosphate
-
 Tequatrovirus T4 adenosine 5'-triphosphate derivative of tripolyphosphate + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
T4 DNA ligase
-
 Tequatrovirus T4

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0006
-
 methylenebisphosphonate in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C Tequatrovirus T4
0.0041
-
 clodronate in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C Tequatrovirus T4
0.0062
-
 tripolyphosphate in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C Tequatrovirus T4
0.07
-
 etidronate in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C Tequatrovirus T4

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Tequatrovirus T4