Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DLT1270/pRARE-2 cells | Thermococcus sp. |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 35% (v/v) tacsimate pH 7.0 as a precipitant | Thermococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sp. | C0LJI8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography, Mono beads Q column chromatography, and Superdex 200 gel filtration | Thermococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Thermococcus sp. | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent DNA ligase | - |
Thermococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermococcus sp. |