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Literature summary for 6.5.1.1 extracted from

  • Bezsudnova, E.Y.; Kovalchuk, M.V.; Mardanov, A.V.; Poliakov, K.M.; Popov, V.O.; Ravin, N.V.; Skryabin, K.G.; Smagin, V.A.; Stekhanova, T.N.; Tikhonova, T.V.
    Overexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519 (2009), Acta Crystallogr. Sect. F, 65, 368-371.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DLT1270/pRARE-2 cells Thermococcus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 35% (v/v) tacsimate pH 7.0 as a precipitant Thermococcus sp.

Organism

Organism UniProt Comment Textmining
Thermococcus sp. C0LJI8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography, Mono beads Q column chromatography, and Superdex 200 gel filtration Thermococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Thermococcus sp. AMP + diphosphate + (deoxyribonucleotide)n+m
-
?

Synonyms

Synonyms Comment Organism
ATP-dependent DNA ligase
-
Thermococcus sp.

Cofactor

Cofactor Comment Organism Structure
ATP
-
Thermococcus sp.