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Literature summary for 6.5.1.1 extracted from

  • Horspool, D.R.; Coope, R.J.; Holt, R.A.
    Efficient assembly of very short oligonucleotides using T4 DNA ligase (2010), BMC Res. Notes, 3, 291.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ligation by T4 DNA ligase is dependent on the formation of a double stranded DNA duplex of at least five base pairs surrounding the site of ligation. However, ligations can be performed effectively with overhangs smaller than five base pairs and oligonucleotides as small as octamers, in the presence of a second, complementary oligonucleotide Tequatrovirus T4 ?
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Synonyms

Synonyms Comment Organism
T4 DNA ligase
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Tequatrovirus T4

Cofactor

Cofactor Comment Organism Structure
ATP
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Tequatrovirus T4