Crystallization (Comment) | Organism |
---|---|
1.8 A resolution structure of Pyrococcus furiosus DNA ligase. The enzyme comprises the N-terminal DNA binding domain, the middle adenylation domain, and the C-terminal OB-fold domain. The architecture of each domain resembles those of human DNA ligase I, but the domain arrangements differ strikingly between the two enzymes | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
K534A | the wild-type R531A and mutant K534A enzymes exhibit almost the same DNA ligation activities both in the presence and in the absence of externally added ATP, contains AMP in the crystal | Pyrococcus furiosus |
R531A | the wild-type R531A and mutant K534A enzymes exhibit almost the same DNA ligation activities both in the presence and in the absence of externally added ATP, contains AMP in the crystal | Pyrococcus furiosus |
R531A/K534A | the mutations of both basic residues (R531A and K534A) severely affected the ligation activity, especially in the absence of ATP, does not contain AMP in the crystal | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | P56709 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
PfuLig | - |
Pyrococcus furiosus |