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Literature summary for 6.5.1.2 extracted from
- DNA ligases from thermophilic bacteria enhance PCR amplification of long DNA sequences (2009), Biochemistry, 74, 557-561.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Thermus aquaticus |
| expressed in Escherichia coli | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Thermus aquaticus | - |
AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m | - |
? |  |
| NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Thermus thermophilus | - |
AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus aquaticus | - |
- |
- |
| Thermus thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE column chromatography and phosphor-cellulose column chromatography | Thermus thermophilus |
| DEAE column chromatographyand phosphor-cellulose column chromatography | Thermus aquaticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Thermus aquaticus | AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m | - |
? | |
| NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Thermus thermophilus | AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAD+-dependent DNA ligase | - |
Thermus aquaticus |
| NAD+-dependent DNA ligase | - |
Thermus thermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | NAD+ presence is essential for exhibiting DNA ligase activity | Thermus aquaticus | |
| NAD+ | NAD+ presence is essential for exhibiting DNA ligase activity | Thermus thermophilus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | DNA ligase enhances efficiency of DNA polymerase in long PCR amplification | Thermus aquaticus |
| physiological function | DNA ligase enhances efficiency of DNA polymerase in long PCR amplification | Thermus thermophilus |
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