Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Thermus aquaticus |
expressed in Escherichia coli | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Thermus aquaticus | - |
AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Thermus thermophilus | - |
AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus aquaticus | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE column chromatography and phosphor-cellulose column chromatography | Thermus thermophilus |
DEAE column chromatographyand phosphor-cellulose column chromatography | Thermus aquaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Thermus aquaticus | AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Thermus thermophilus | AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent DNA ligase | - |
Thermus aquaticus |
NAD+-dependent DNA ligase | - |
Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+ presence is essential for exhibiting DNA ligase activity | Thermus aquaticus | |
NAD+ | NAD+ presence is essential for exhibiting DNA ligase activity | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
physiological function | DNA ligase enhances efficiency of DNA polymerase in long PCR amplification | Thermus aquaticus |
physiological function | DNA ligase enhances efficiency of DNA polymerase in long PCR amplification | Thermus thermophilus |