General Stability | Organism |
---|---|
not affected by several cycles of freezing and thawing | Homo sapiens |
resistant to protease digestion | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Homo sapiens | |
NEM | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
ATP | - |
Homo sapiens | |
0.006 | - |
ATPgammaS | ATP | Homo sapiens | |
0.2 | - |
GTP | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | only 5% of the activation by Mg2+ | Homo sapiens | |
K+ | optimal concentration: 150-200 mM | Homo sapiens | |
Mg2+ | required | Homo sapiens | |
Mg2+ | maximal activity at 4-5 mM | Homo sapiens | |
Mn2+ | only 5% of the activation by Mg2+ | Homo sapiens | |
Na+ | optimal concentration: 150-200 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | 40000 | glycerol density gradient sedimentation, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + [RNA]-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside | formation of a covalent cyclase-AMP intermediate | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, stable for at least 6 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA 3'-terminal-phosphate | no activity with dATP | Homo sapiens | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
ATPgammaS + RNA 3'-terminal-phosphate | - |
Homo sapiens | AMP + thiodiphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
CTP + RNA 3'-terminal-phosphate | - |
Homo sapiens | CMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
GTP + RNA 3'-terminal-phosphate | - |
Homo sapiens | GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
UTP + RNA 3'-terminal-phosphate | - |
Homo sapiens | UMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000-40000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
similar rates of reaction at 25°C, 30°C, and 37°C | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
37 | 0°C: 25% of maximal activity, 12°C: 60% of maximal activity, 25-37°C: maximal activity | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
5 min, 55% loss of activity | Homo sapiens |
50 | - |
5 min, 95% loss of activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Homo sapiens |