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Literature summary for 6.5.1.4 extracted from

  • Filipowicz, W.; Vicente, O.
    RNA 3'-terminal phosphate cyclase from HeLa cells (1990), Methods Enzymol., 181, 499-510.
    View publication on PubMed

General Stability

General Stability Organism
not affected by several cycles of freezing and thawing Homo sapiens
resistant to protease digestion Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Homo sapiens
NEM
-
Homo sapiens
Vanadyl-ribonucleoside complex
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
ATP
-
Homo sapiens
0.006
-
ATPgammaS ATP Homo sapiens
0.2
-
GTP
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Homo sapiens 5634
-
nucleus
-
Xenopus laevis 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ only 5% of the activation by Mg2+ Homo sapiens
K+ optimal concentration: 150-200 mM Homo sapiens
Mg2+ maximal activity at 4-5 mM Homo sapiens
Mn2+ only 5% of the activation by Mg2+ Homo sapiens
Na+ optimal concentration: 150-200 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000 40000 glycerol density gradient sedimentation, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Xenopus laevis it was suggested that the enzyme could be responsible for the generation of the 2',3'-cyclic termini required for ligation of RNA by eukaryotic ligases and, consequently, be involved in tRNA slicing. It was found, however, that the slicing endonucleases responsible for the excision of introns from tRNA precursors in yeast and Xenopus sp. directly produce RNA molecules with cyclic phosphate ends ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-
oocyte
-
Xenopus laevis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, stable for at least 6 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA 3'-terminal-phosphate
-
Homo sapiens AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?
ATP + RNA 3'-terminal-phosphate
-
Xenopus laevis AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?
ATPgammaS + RNA 3'-terminal-phosphate
-
Homo sapiens AMP + thiodiphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?
CTP + RNA 3'-terminal-phosphate reaction proceeds 15-20 times slower than in presence of ATP Homo sapiens CMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?
GTP + RNA 3'-terminal-phosphate reaction proceeds 15-20 times slower than in presence of ATP Homo sapiens GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?
additional information it was suggested that the enzyme could be responsible for the generation of the 2',3'-cyclic termini required for ligation of RNA by eukaryotic ligases and, consequently, be involved in tRNA slicing. It was found, however, that the slicing endonucleases responsible for the excision of introns from tRNA precursors in yeast and Xenopus sp. directly produce RNA molecules with cyclic phosphate ends Xenopus laevis ?
-
?
UTP + RNA 3'-terminal-phosphate reaction proceeds 15-20 times slower than in presence of ATP Homo sapiens UMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 38000-40000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
similar rates of reaction at 25°C, 30°C, and 37°C Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
37 0°C: 25% of maximal activity, 12°C: 60% of maximal activity, 25-37°C: maximal activity Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
3 d, 50% loss of activity Homo sapiens
20
-
3-4 h, 50% loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
8 10.5 8.0-9.0: maximal activity, 10.5: 70% of maximal activity Homo sapiens