Cloned (Comment) | Organism |
---|---|
expression of domain I in Escherichia coli | Rhodospirillum rubrum |
expression of wild-type domain III, T393C, R425C, G430C and A432C mutant domain III in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A432C | mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 150% higher reaction rate than wild-type domain III/R. rubrum domain I mixture | Escherichia coli |
G430C | mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 850% higher reaction rate than wild-type domain III/R. rubrum domain I mixture | Escherichia coli |
R425C | mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 425% higher reaction rate than wild-type domain III/R. rubrum domain I mixture | Escherichia coli |
T393C | mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 175% higher reaction rate than wild-type domain III/R. rubrum domain I mixture | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant domain I | Rhodospirillum rubrum |
recombinant wild-type, T393C, R425C, G430C and A432C mutant domain III | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0667 | - |
reduced acetylpyridine adenine dinucleotide | reverse reaction of wild-type domain III/R. rubrum domain I mixture | Escherichia coli | |
0.1 | - |
reduced acetylpyridine adenine dinucleotide | reverse reaction of A432C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
0.117 | - |
reduced acetylpyridine adenine dinucleotide | reverse reaction of D393C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
0.167 | - |
reduced acetylpyridine adenine dinucleotide | reverse reaction of H345C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
0.167 | - |
reduced acetylpyridine adenine dinucleotide | reverse reaction of R350C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
0.283 | - |
reduced acetylpyridine adenine dinucleotide | reverse reaction of K424C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
0.283 | - |
reduced acetylpyridine adenine dinucleotide | reverse reaction of R425C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
0.55 | - |
reduced acetylpyridine adenine dinucleotide | reverse reaction of A348C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
0.567 | - |
reduced acetylpyridine adenine dinucleotide | reverse reaction of G430C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
0.917 | - |
reduced acetylpyridine adenine dinucleotide | reverse reaction of D392C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
10 | - |
reduced acetylpyridine adenine dinucleotide | cyclic reaction of R425C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
11.7 | - |
reduced acetylpyridine adenine dinucleotide | cyclic reaction of G430C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
15 | - |
reduced acetylpyridine adenine dinucleotide | cyclic reaction of D392C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
20.8 | - |
reduced acetylpyridine adenine dinucleotide | cyclic reaction of H345C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
33.3 | - |
reduced acetylpyridine adenine dinucleotide | cyclic reaction of R350C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
36.7 | - |
reduced acetylpyridine adenine dinucleotide | cyclic reaction of K424C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
40 | - |
reduced acetylpyridine adenine dinucleotide | cyclic reaction of D393C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
51.7 | - |
reduced acetylpyridine adenine dinucleotide | cyclic reaction of A432C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
55 | - |
reduced acetylpyridine adenine dinucleotide | cyclic reaction of A348C mutant domain III/R. rubrum domain I mixture | Escherichia coli | |
81.7 | - |
reduced acetylpyridine adenine dinucleotide | cyclic reaction of wild-type domain III/R. rubrum domain I mixture | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NAD+ | - |
Rhodospirillum rubrum | |
NADH | - |
Escherichia coli | |
NADH | - |
Rhodospirillum rubrum | |
NADP+ | - |
Escherichia coli | |
NADP+ | - |
Rhodospirillum rubrum | |
NADPH | - |
Escherichia coli | |
NADPH | - |
Rhodospirillum rubrum |