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Literature summary for 7.1.1.1 extracted from

  • Bizouarn, T.; van Boxel, G.I.; Bhakta, T.; Jackson, J.B.
    Nucleotide binding affinities of the intact proton-translocating transhydrogenase from Escherichia coli (2005), Biochim. Biophys. Acta, 1708, 404-410.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JM109 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Escherichia coli 16020
-
membrane the enzyme is composed of three components. The dI and dIII components, which house the binding site for NAD(H) and NADP(H), respectively, are peripheral to the membrane, and dII spans the membrane Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H+/out + NADH + NADP+ Escherichia coli the enzyme couples the redox reaction between NAD(H) and NADP(H) to the transport of protons across a membrane H+/in + NAD+ + NADPH
-
?
NADPH + NAD+ + H+[side 1] Escherichia coli
-
NADP+ + NADH + H+[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
purified enzyme, at pH 7.0 and 20°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H+/out + NADH + NADP+ the enzyme couples the redox reaction between NAD(H) and NADP(H) to the transport of protons across a membrane Escherichia coli H+/in + NAD+ + NADPH
-
?
NADPH + NAD+ + H+[side 1]
-
Escherichia coli NADP+ + NADH + H+[side 2]
-
?
NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1]
-
Escherichia coli NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2]
-
r

Synonyms

Synonyms Comment Organism
proton-translocating transhydrogenase
-
Escherichia coli