Crystallization (Comment) | Organism |
---|---|
mutant enzymes are crystallized by the hanging drop vapor diffusion method, using in 16-24% (w/v) 8K-PEG, 20-150 mM (NH4)2SO4, 100mM MES, pH 6.0, and 10% (v/v) glycerol in the presence of 50 mM NAD+ and 5 mM NADP+ | Rhodospirillum rubrum |
Protein Variants | Comment | Organism |
---|---|---|
D135N | mutation has no effect in binding affinity of either NAD+ or NADH | Rhodospirillum rubrum |
D135N | the mutant shows reduced activity compared to the wild type enzyme | Rhodospirillum rubrum |
E155W | the mutant shows reduced activity compared to the wild type enzyme | Rhodospirillum rubrum |
Q132N | the mutant shows reduced activity compared to the wild type enzyme | Rhodospirillum rubrum |
R127A | mutation strongly inhibits the rate of transhydrogenation and alters the nucleotide-binding properties of the dI protein. When dIR127A is reconstituted into the intact enzyme in membranes, transhydrogenation rates are negligible. dI is the NAD(H)-binding component of the transhydrogenase | Rhodospirillum rubrum |
R127A | the mutant shows reduced activity compared to the wild type enzyme | Rhodospirillum rubrum |
R127M | mutation strongly inhibits the rate of transhydrogenation and alters the nucleotide-binding properties of the dI protein. When dIR127M is reconstituted into the intact enzyme in membranes, transhydrogenation rates are negligible. dI is the NAD(H)-binding component of the transhydrogenase | Rhodospirillum rubrum |
R127M | the mutant shows reduced activity compared to the wild type enzyme | Rhodospirillum rubrum |
S135A | mutation has no effect in binding affinity of either NAD+ or NADH | Rhodospirillum rubrum |
S138A | the mutant shows reduced activity compared to the wild type enzyme | Rhodospirillum rubrum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rhodospirillum rubrum | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Rhodospirillum rubrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ + H+[side 1] | Rhodospirillum rubrum | - |
NADP+ + NADH + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ + H+[side 1] | - |
Rhodospirillum rubrum | NADP+ + NADH + H+[side 2] | - |
? | |
NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1] | - |
Rhodospirillum rubrum | NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2] | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 40000, SDS-PAGE | Rhodospirillum rubrum |
Synonyms | Comment | Organism |
---|---|---|
transhydrogenase | - |
Rhodospirillum rubrum |