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Literature summary for 7.1.1.1 extracted from

  • Glavas, N.A.; Hou, C.; Bragg, P.D.
    Involvement of histidine-91 of the beta subunit in proton translocation by the pyridine nucleotide transhydrogenase of Escherichia coli (1995), Biochemistry, 34, 7694-7702.
    View publication on PubMed
Search for more literature for 7.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM 109 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
H91C the mutant of the beta subunit is unable to undergo the conformational change that occurs on binding of the substrates NADP+ or NADPH. The mutant retains 12% of the hydride transfer activity while proton translocation is reduced to 7% compared to the wild type enzyme Escherichia coli
H91D the mutant of the beta subunit retains 15% of the hydride transfer activity while proton translocation is reduced to 9% compared to the wild type enzyme Escherichia coli
H91K the mutant of the beta subunit is present in the NADP(H)-induced conformation even in the absence of these substrates. The mutant retains 4% of the hydride transfer activity while proton translocation is reduced to 20% compared to the wild type enzyme Escherichia coli
H91N the mutant of the beta subunit retains 80% of the hydride transfer activity while proton translocation is reduced to 7% compared to the wild type enzyme Escherichia coli
H91S the mutant of the beta subunit is unable to undergo the conformational change that occurs on binding of the substrates NADP+ or NADPH. The mutant retains 19% of the hydride transfer activity while proton translocation is reduced to 11% compared to the wild type enzyme Escherichia coli
H91T the mutant of the beta subunit is unable to undergo the conformational change that occurs on binding of the substrates NADP+ or NADPH. The mutant retains 11% of the hydride transfer activity while proton translocation is reduced to 8% compared to the wild type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
N,N'-dicyclohexylcarbodiimide about 12% residual activity after 180 min at 0.5 mM Escherichia coli
N-cyclohexyl-N'-(4-dimethylaminonaphthyl)carbodiimide about 25% residual activity after 200 min at 0.5 mM Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADPH + NAD+ + H+[side 1] Escherichia coli
-
 NADP+ + NADH + H+[side 2]
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + NAD+ + H+[side 1]
-
 Escherichia coli NADP+ + NADH + H+[side 2]
-
 r

Synonyms

Synonyms Comment Organism
pyridine nucleotide transhydrogenase
-
 Escherichia coli