Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C600 and BL21(DE3) cells | Rhodospirillum rubrum |
Protein Variants | Comment | Organism |
---|---|---|
E155W | the mutant shows wild type activity | Rhodospirillum rubrum |
E155W/R165A | the mutations mutation do not significantly affect catalytic activity | Rhodospirillum rubrum |
R165A | a higher concentration of the nucleotide is needed to achieve the half-maximal rate compared to with the wild type protein | Rhodospirillum rubrum |
W72F | the mutant shows wild type activity | Rhodospirillum rubrum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rhodospirillum rubrum | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ + H+/in | Rhodospirillum rubrum | - |
NADP+ + NADH + H+/out | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ + H+/in | - |
Rhodospirillum rubrum | NADP+ + NADH + H+/out | - |
r | |
NADPH + oxidized acetyl pyridine adenine dinucleotide + H+/in | - |
Rhodospirillum rubrum | NADP+ + reduced acetyl pyridine adenine dinucleotide + H+/out | - |
r |
Synonyms | Comment | Organism |
---|---|---|
proton-translocating transhydrogenase | - |
Rhodospirillum rubrum |