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Literature summary for 7.1.1.1 extracted from

  • Huxley, L.; Quirk, P.G.; Cotton, N.P.J.; White, S.A.; Jackson, J.B.
    The specificity of proton-translocating transhydrogenase for nicotinamide nucleotides (2011), Biochim. Biophys. Acta, 1807, 85-94.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C600 and BL21(DE3) cells Rhodospirillum rubrum

Protein Variants

Protein Variants Comment Organism
E155W the mutant shows wild type activity Rhodospirillum rubrum
E155W/R165A the mutations mutation do not significantly affect catalytic activity Rhodospirillum rubrum
R165A a higher concentration of the nucleotide is needed to achieve the half-maximal rate compared to with the wild type protein Rhodospirillum rubrum
W72F the mutant shows wild type activity Rhodospirillum rubrum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Rhodospirillum rubrum 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADPH + NAD+ + H+/in Rhodospirillum rubrum
-
NADP+ + NADH + H+/out
-
r

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + NAD+ + H+/in
-
Rhodospirillum rubrum NADP+ + NADH + H+/out
-
r
NADPH + oxidized acetyl pyridine adenine dinucleotide + H+/in
-
Rhodospirillum rubrum NADP+ + reduced acetyl pyridine adenine dinucleotide + H+/out
-
r

Synonyms

Synonyms Comment Organism
proton-translocating transhydrogenase
-
Rhodospirillum rubrum