KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
NADH | with ubiquinone-6 as acceptor | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 0.08 mol Fe per mol of flavin after dialysis | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
rotenone-insensitive enzyme | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
61.9 | - |
reaction with ubiquinone-6 | Saccharomyces cerevisiae |
1671 | - |
reaction with ubiquinone-2 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + ubiquinone | - |
Saccharomyces cerevisiae | NAD+ + ubiquinol | - |
? | |
NADH + ubiquinone-2 | - |
Saccharomyces cerevisiae | NAD+ + reduced ubiquinol-2 | - |
? | |
NADH + ubiquinone-6 | - |
Saccharomyces cerevisiae | NAD+ + ubiquinol-6 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
80 | 100 | NADH | with ubiquinone-6 as acceptor, Triton assay medium | Saccharomyces cerevisiae | |
500 | 550 | NADH | with ubiquinone-6 as acceptor, mitochondria | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | reaction with ubiquinone-6 is almost constant between pH 4.5 and pH 9.5 | Saccharomyces cerevisiae |
6.2 | - |
reaction with ubiquinone-2 | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
activity is irreversibly destroyed below | Saccharomyces cerevisiae |
9.5 | - |
activity is irreversibly destroyed above | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains a single molecule of FAD per subunit | Saccharomyces cerevisiae | |
NADH | - |
Saccharomyces cerevisiae |