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Literature summary for 7.1.1.2 extracted from

  • Sherwood, S.; Hirst, J.
    Investigation of the mechanism of proton translocation by NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria: does the enzyme operate by a Q-cycle mechanism? (2006), Biochem. J., 400, 541-550.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.1.2

Inhibitors

Inhibitors Comment Organism Structure
Piericidin A 0.0023 mM Bos taurus
rotenone 0.00025 mM Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0204
-
 ubiquinone-2 using 0.1 mM NADH at pH 7.5 Bos taurus
0.024
-
 decylubiquinone using 0.1 mM NADH at pH 7.5 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion the enzyme oxidizes NADH in the mitochondrial matrix and reduces ubiquinone in the inner mitochondrial membrane Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe-S contains two [2Fe-2S] clusters and six [4Fe-4S] clusters Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephacryl S-300 gel-filtration Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + decylubiquinone
-
 Bos taurus NAD+ + decylubiquinol
-
 ?
NADH + ubiquinone-2
-
 Bos taurus NAD+ + ubiquinol-2
-
 ?

Subunits

Subunits Comment Organism
More consists of 45 different subunits Bos taurus

Synonyms

Synonyms Comment Organism
complex I
-
 Bos taurus
NADH:ubiquinone oxidoreductase
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Bos taurus

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Bos taurus
NADH
-
 Bos taurus