Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.1.1.2 extracted from

  • Dong, C.K.; Patel, V.; Yang, J.C.; Dvorin, J.D.; Duraisingh, M.T.; Clardy, J.; Wirth, D.F.
    Type II NADH dehydrogenase of the respiratory chain of Plasmodium falciparum and its inhibitors (2009), Bioorg. Med. Chem. Lett., 19, 972-975.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged NDH2 in Escherichia coli Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition: dibenziodolium chloride, diphenyliodonium chloride, 1-hydroxy-2-dodecyl-4(1H)quinolone, atovaquone, antimycin A, rotenone, flavone, artemisinin Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 ubiquinone-1 pH 8.0, 25°C Plasmodium falciparum
0.0046
-
 oxidized dichlorophenolindophenol pH 8.0, 25°C Plasmodium falciparum
0.104
-
 ubiquinone-0 pH 8.0, 25°C Plasmodium falciparum
0.239
-
 menadione pH 8.0, 25°C Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe signal shape and g values of cluster N4 [4Fe(G)C], are particularly sensitive to the changes of the environment around N4 Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged pfNDH2 Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with coenzyme Q4 and coenzyme QD Plasmodium falciparum ?
-
 ?
NADH + H+ + menadione
-
 Plasmodium falciparum NAD+ + menadiol
-
 ?
NADH + H+ + oxidized dichlorophenolindophenol
-
 Plasmodium falciparum NAD+ + reduced dichlorophenolindophenol
-
 ?
NADH + H+ + ubiquinone-0 prefers ubiquinol-0 as an acceptor substrate, and can also use the artificial electron acceptors, menadione and dichlorophenol–indophenol Plasmodium falciparum NAD+ + ubiquinol-0
-
 ?
NADH + H+ + ubiquinone-1
-
 Plasmodium falciparum NAD+ + ubiquinol-1
-
 ?

Synonyms

Synonyms Comment Organism
NDH2
-
 Plasmodium falciparum
PfNdh2
-
 Plasmodium falciparum
type II NADH dehydrogenase
-
 Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
 ubiquinone-1 pH 8.0, 25°C Plasmodium falciparum
0.032
-
 oxidized dichlorophenolindophenol pH 8.0, 25°C Plasmodium falciparum
0.077
-
 menadione pH 8.0, 25°C Plasmodium falciparum
0.33
-
 ubiquinone-0 pH 8.0, 25°C Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
 Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0: about 50% of maximal activity, pH 10.0: about 50% of maximal activity Plasmodium falciparum