Any feedback?
Literature summary for 7.1.1.2 extracted from
- On the mechanism of rotenone-insensitive reduction of quinones by mitochondrial NADH:ubiquinone reductase. The high affinity binding of NAD+ and NADH to the reduced enzyme form (1991), FEBS Lett., 284, 192-194.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP-ribose | ADP-ribose acts as mixed-type inhibitors for NADH, ferricyanide and 5,8-dioxy-1,4-naphthoquinone | Bos taurus |  |
| arylazido-beta-alanyl-NAD+ | competitive inhibition towards NADH and ferricyanide | Bos taurus | |
| additional information | the oxidation of NADH by 5,8-dioxy-1,4-naphthoquinone, catalyzed by complex I is completely insensitive to 0.002 mM rotenone | Bos taurus | |
| NAD+ | NAD+ acts as mixed-type inhibitors for NADH, ferricyanide and 5,8-dioxy-1,4-naphthoquinone. At saturating concentrations of oxidizer, NAD+ acts as a competitive inhibitor for NADH | Bos taurus | |
| NADH | NADH acts as a competitive inhibitor for 5,8-dioxy-1,4-naphthoquinone, NADH acts as a linear competitive inhibitor for ferricyanide | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Bos taurus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricyanide + NADH | - |
Bos taurus | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| NADH + H+ + 5,8-dioxo-1,4-naphthoquinone | - |
Bos taurus | NAD+ + 5,8-dioxo-1,4-naphthoquinol | - |
? | |
| NADH + H+ + ubiquinone | - |
Bos taurus | NAD+ + ubiquinol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| complex I | - |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Bos taurus | |
| iron-sulfur centre | - |
Bos taurus | |
| NADH | - |
Bos taurus | |
| ubiquinone | - |
Bos taurus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
NADH | in 0.4 M phosphate, using 5,8-dioxy-1,4-naphthoquinone as substrate, at pH 7.0, 25°C | Bos taurus | |
| 0.05 | - |
NADH | in 0.4 M phosphate, using ferricyanide as substrate, at pH 7.0, 25°C | Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
