Literature summary for 7.1.1.2 extracted from

Jones, A.J.; Blaza, J.N.; Bridges, H.R.; May, B.; Moore, A.L.; Hirst, J.
A self-assembled respiratory chain that catalyzes NADH oxidation by ubiquinone-10 cycling between complex I and the alternative oxidase (2016), Angew. Chem. Int. Ed. Engl., 55, 728-731 .

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Application

Application	Comment	Organism
analysis	kinetic analysis of enzyme using a seff-assembled proteoliposome system containing complex I, Q10, and quinol oxidase (the alternative oxidase, AOX) to recycle Q10H2 to Q10. Complex I is completely rate determining and the Q10 pool is kept oxidized under steady-state catalysis	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	Ubiquinone-10	KM value for complex I is 3.9 nmol/mg phospholipid	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Bos taurus	5739	-

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
heart	-	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
18.6	-	pH not specified in the publication, temperature not specified in the publication	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADH + ubiquinone-10 + 5 H+[side 1]	-	Bos taurus	NAD+ + ubiquinol-10 + 4 H+[side 2]	-	?

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Release 2023.1 (January 2023)