BRENDA - Enzyme Database show
show all sequences of 7.1.1.3

Site-directed mutants of the cytochrome bo ubiquinol oxidase of Escherichia coli: amino acid substitutions for two histidines that are putative CuB ligands

Calhoun, M.W.; Hill, J.J.; Lemieux, L.J.; Ingledew, W.J.; Alben, J.O.; Gennis, R.B.; Biochemistry 32, 11524-11529 (1993)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
H333C
nonfunctional enzyme
Escherichia coli
H333L
the mutation eliminates the magnetic coupling between heme o and CuB leading to a nonfunctional enzyme
Escherichia coli
H333N
nonfunctional enzyme
Escherichia coli
H333Q
nonfunctional enzyme
Escherichia coli
H334L
the mutation eliminates the magnetic coupling between heme o and CuB leading to a nonfunctional enzyme
Escherichia coli
H334M
nonfunctional enzyme
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
copper
the high-spin heme is magnetically coupled to a copper, CuB, forming a binuclear center which is the site of oxygen reduction to water
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli GLlOl
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
heme
the high-spin heme is magnetically coupled to a copper, CuB, forming a binuclear center which is the site of oxygen reduction to water
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
the high-spin heme is magnetically coupled to a copper, CuB, forming a binuclear center which is the site of oxygen reduction to water
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H333C
nonfunctional enzyme
Escherichia coli
H333L
the mutation eliminates the magnetic coupling between heme o and CuB leading to a nonfunctional enzyme
Escherichia coli
H333N
nonfunctional enzyme
Escherichia coli
H333Q
nonfunctional enzyme
Escherichia coli
H334L
the mutation eliminates the magnetic coupling between heme o and CuB leading to a nonfunctional enzyme
Escherichia coli
H334M
nonfunctional enzyme
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
copper
the high-spin heme is magnetically coupled to a copper, CuB, forming a binuclear center which is the site of oxygen reduction to water
Escherichia coli
Other publictions for EC 7.1.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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13
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1
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19
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13
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1
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1
-
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5
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1
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1
1
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742754
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Location of the substrate bin ...
Escherichia coli
J. Am. Chem. Soc.
139
8346-8354
2017
-
-
1
-
15
-
2
11
-
1
-
1
-
2
-
-
-
-
-
-
-
-
3
-
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-
-
-
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-
1
2
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-
-
1
1
-
15
-
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2
2
11
-
1
-
1
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-
3
-
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741935
Sun
Q-band electron-nuclear doubl ...
Escherichia coli
Biochemistry
55
5714-5725
2016
-
-
1
-
-
-
-
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1
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1
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2
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1
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1
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1
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743301
Korshunov
The cytochrome bd oxidase of ...
Escherichia coli
Mol. Microbiol.
101
62-77
2016
-
-
-
-
-
-
1
-
-
-
-
1
-
2
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1
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1
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1
1
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1
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1
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1
1
-
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743596
Leclerc
The cytochrome bd oxidase of ...
Porphyromonas gingivalis, Porphyromonas gingivalis ATCC 33277
PLoS ONE
10
e0143808
2015
-
-
-
-
-
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14
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1
1
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743792
Lu
The cytochrome bd-type quinol ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc(2)155
Sci. Rep.
5
10333
2015
-
-
-
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2
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10
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2
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1
1
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741957
Giuffre
Cytochrome bd oxidase and bac ...
Escherichia coli
Biochim. Biophys. Acta
1837
1178-1187
2014
-
-
-
-
-
-
1
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1
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1
-
3
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1
1
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1
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1
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1
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1
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742510
Hoeser
Subunit CydX of Escherichia c ...
Escherichia coli
FEBS Lett.
588
1537-1541
2014
-
-
1
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1
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1
1
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2
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1
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1
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1
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742946
Liu
Improved poly(3-hydroxybutyra ...
Escherichia coli, Escherichia coli JM109
J. Biotechnol.
192
170-176
2014
-
1
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2
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32
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725289
Richhardt
Evidence for a key role of cyt ...
Gluconobacter oxydans
J. Bacteriol.
195
4210-4220
2013
-
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3
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723938
Yildiz
Cell-free synthesis of cytochr ...
Escherichia coli
Anal. Biochem.
423
39-45
2012
-
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1
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1
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1
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1
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1
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1
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714331
Egawa
Differential effects of glutam ...
Escherichia coli, Escherichia coli C43 (DE3)
Biochim. Biophys. Acta
1807
1342-1348
2011
-
-
-
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1
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4
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1
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2
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1
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1
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1
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1
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2
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714308
Yap
The quinone-binding sites of t ...
Escherichia coli
Biochim. Biophys. Acta
1797
1924-1932
2010
-
-
-
-
12
-
2
4
1
1
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2
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1
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1
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2
4
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2
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12
-
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2
4
4
1
1
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1
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-
1
-
-
-
-
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715509
Shepherd
Compensations for diminished t ...
Escherichia coli, Escherichia coli BW25113
J. Biol. Chem.
285
18464-18472
2010
-
-
-
-
-
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29
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1
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1
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2
2
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714169
Egawa
Communication between R481 and ...
Escherichia coli
Biochemistry
48
12113-12124
2009
-
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2
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1
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2
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1
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1
1
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716735
Belevich
Time-resolved electrometric an ...
Escherichia coli, Escherichia coli GO105
Proc. Natl. Acad. Sci. USA
102
3657-3662
2005
-
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1
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4
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1
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2
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715458
Park
Vitreoscilla hemoglobin binds ...
Escherichia coli, Escherichia coli JM103, Pseudomonas aeruginosa, Vitreoscilla sp.
J. Biol. Chem.
277
33334-33337
2002
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3
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9
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713612
Abramson
Purification, crystallization ...
Escherichia coli, Escherichia coli GO105
Acta Crystallogr. Sect. D
56
1076-1078
2000
-
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1
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5
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1
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716311
Abramson
The structure of the ubiquinol ...
Escherichia coli
Nat. Struct. Biol.
7
910-917
2000
-
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1
5
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5
1
1
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2
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1
1
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1
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1
1
5
-
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5
1
1
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-
1
1
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1
1
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715003
Mogi
Role of a bound ubiquinone on ...
Escherichia coli, Escherichia coli GO103/pHN3795-1
FEBS Lett.
457
223-226
1999
-
-
-
-
-
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3
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1
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2
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2
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2
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1
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2
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714141
Mogi
Substitutions of conserved aro ...
Escherichia coli, Escherichia coli GO103/pMFO2
Biochemistry
37
1632-1639
1998
-
-
-
-
16
-
-
-
1
1
-
-
-
3
-
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-
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-
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-
2
1
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-
-
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1
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1
-
16
-
-
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1
1
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-
2
1
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-
-
-
-
-
-
1
1
-
-
-
714139
Ek
Oxidation of ubiquinol by cyto ...
Escherichia coli
Biochemistry
36
5425-5431
1997
-
-
-
-
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2
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1
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1
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1
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1
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1
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-
-
-
715000
Tsubaki
Glutamate-286 mutants of cytoc ...
Escherichia coli, Escherichia coli GO103
FEBS Lett.
416
247-250
1997
-
-
-
-
3
-
1
-
1
1
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-
4
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4
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-
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2
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2
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3
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1
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1
1
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-
-
4
-
-
-
-
-
-
-
-
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-
1
1
-
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-
715399
Kawasaki
Substitutions of charged amino ...
Escherichia coli, Escherichia coli ST4676
J. Biochem.
122
422-429
1997
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-
-
-
15
-
-
-
2
1
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3
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2
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1
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1
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15
-
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2
1
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-
2
-
-
-
-
-
-
-
-
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1
1
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714135
Garcia-Horsman
Proton transfer in cytochrome ...
Escherichia coli
Biochemistry
34
4428-4433
1995
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-
1
-
21
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1
1
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2
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1
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1
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1
1
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21
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1
1
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-
1
-
-
-
-
-
-
-
-
-
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1
1
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-
-
714997
Orii
Facilitated intramolecular ele ...
Escherichia coli, Escherichia coli GO103
FEBS Lett.
352
151-154
1994
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-
-
-
-
-
-
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-
1
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3
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1
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2
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2
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1
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1
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-
-
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-
714130
Thomas
Substitution of asparagine for ...
Escherichia coli, Escherichia coli GL101
Biochemistry
32
10923-10928
1993
-
-
-
-
7
-
-
-
1
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-
-
-
6
-
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2
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7
-
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-
1
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-
-
2
-
-
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-
-
-
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-
-
714131
Thomas
Site-directed mutagenesis of h ...
Escherichia coli, Escherichia coli GL101
Biochemistry
32
11173-11180
1993
-
-
1
-
10
-
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-
1
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4
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2
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-
1
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1
1
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10
-
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1
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-
-
2
-
-
-
-
-
-
-
-
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-
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-
-
-
-
714132
Calhoun
Site-directed mutants of the c ...
Escherichia coli, Escherichia coli GLlOl
Biochemistry
32
11524-11529
1993
-
-
-
-
6
-
-
-
-
1
-
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-
4
-
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-
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-
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-
1
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1
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6
-
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1
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-
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-
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-
-
-
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-
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-
714285
Miyoshi-Akiyama
Purification and properties of ...
Vibrio alginolyticus
Biochim. Biophys. Acta
1141
283-287
1993
6
-
-
-
-
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3
-
1
1
3
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-
4
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1
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-
-
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4
1
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-
-
-
-
-
-
2
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-
3
6
-
-
2
-
-
-
3
3
-
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1
1
3
-
-
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-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
714286
Bolgiano
Reactions of the membrane-boun ...
Escherichia coli, Escherichia coli RG145
Biochim. Biophys. Acta
1141
95-104
1993
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-
-
-
-
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-
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1
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5
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2
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-
-
2
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-
1
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1
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1
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-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
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-
714129
Minghetti
Modified, large-scale purifica ...
Escherichia coli, Escherichia coli RG145
Biochemistry
31
6917-6924
1992
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-
-
-
-
-
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2
3
4
-
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5
-
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1
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1
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4
1
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1
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1
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2
3
4
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1
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1
-
4
1
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-
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-
-
-
-
-
-
-
-
714885
Salerno
Orientation of the haems of th ...
Escherichia coli, Escherichia coli RG145
Eur. J. Biochem.
198
789-792
1991
-
-
-
-
-
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1
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5
-
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1
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1
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1
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