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Literature summary for 7.1.1.3 extracted from

  • Miyoshi-Akiyama, T.; Hayashi, M.; Unemoto, T.
    Purification and properties of cytochrome bo-type ubiquinol oxidase from a marine bacterium Vibrio alginolyticus (1993), Biochim. Biophys. Acta, 1141, 283-287.
    View publication on PubMed
Search for more literature for 7.1.1.3

Activating Compound

Activating Compound Comment Organism Structure
diphosphatidyl glycerol activates the enzyme with the optimum concentration at 0.04 mg/ml Vibrio alginolyticus
liponox DCH the activity is saturated at 0.01-0.02% (v/v) Liponox DCH Vibrio alginolyticus
additional information the enzyme is not activated by sodium cholate, phosphatidyl glycerol, phosphatidyl ethanolamine and soybean phospholipids Vibrio alginolyticus
n-dodecyl octaethyleneglycol monoether effective activator Vibrio alginolyticus
N-lauroylsarcosine effective activator Vibrio alginolyticus
Zwittergent 3-14 effective activator Vibrio alginolyticus

Inhibitors

Inhibitors Comment Organism Structure
2-n-heptyl-4-hydroxyquinoline N-oxide
-
 Vibrio alginolyticus
KCN
-
 Vibrio alginolyticus
ZnSO4
-
 Vibrio alginolyticus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Vibrio alginolyticus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the oxidase contains copper atoms Vibrio alginolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
 1 * 79000 + 1 * 36000 + 1 * 13000, SDS-PAGE Vibrio alginolyticus
36000
-
 1 * 79000 + 1 * 36000 + 1 * 13000, SDS-PAGE Vibrio alginolyticus
79000
-
 1 * 79000 + 1 * 36000 + 1 * 13000, SDS-PAGE Vibrio alginolyticus

Organism

Organism UniProt Comment Textmining
Vibrio alginolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel gel filtration, DEAE-5PW gel filtration, and Sephacryl S-300 gel filtration Vibrio alginolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
duroquinol + O2 + H+/in
-
 Vibrio alginolyticus duroquinone + H2O + H+/out
-
 ?
additional information horse heart reduced cytochrome c is not oxidized by this enzyme Vibrio alginolyticus ?
-
 ?
N,N,N',N'-tetramethylphenylene diamine + O2
-
 Vibrio alginolyticus ?
-
 ?
ubiquinol-1 + O2 + H+[side 1]
-
 Vibrio alginolyticus ubiquinone-1 + H2O + H+[side 2]
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 79000 + 1 * 36000 + 1 * 13000, SDS-PAGE Vibrio alginolyticus

Synonyms

Synonyms Comment Organism
cytochrome bo-type ubiquinol oxidase
-
 Vibrio alginolyticus

Cofactor

Cofactor Comment Organism Structure
cytochrome the oxidase contains cytochrome b, cytochrome o Vibrio alginolyticus
heme the purified oxidase contains both protoheme and heme O Vibrio alginolyticus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0032
-
 in 25 mM potassium phosphate (pH 7.5), at 30°C Vibrio alginolyticus 2-n-heptyl-4-hydroxyquinoline N-oxide
0.0034
-
 in 25 mM potassium phosphate (pH 7.5), at 30°C Vibrio alginolyticus ZnSO4
0.0064
-
 in 25 mM potassium phosphate (pH 7.5), at 30°C Vibrio alginolyticus KCN

General Information

General Information Comment Organism
physiological function the bo-type ubiquinol oxidase is functioning as a proton pump Vibrio alginolyticus