show reference and datareference onlydata only

Location of the substrate binding site of the cytochrome bo3 ubiquinol oxidase from Escherichia coli

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

2-n-heptyl-4-hydroxyquinoline N-oxide

-

Escherichia coli

AC1-10

-

Escherichia coli

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.016

-

ubiquinol-1

mutant enzyme D75E, at pH 7.0 and 25°C

Escherichia coli

0.024

-

ubiquinol-1

mutant enzyme Q101M, at pH 7.0 and 25°C

Escherichia coli

0.03

-

ubiquinol-1

mutant enzyme L160W, at pH 7.0 and 25°C; mutant enzyme Q101A, at pH 7.0 and 25°C

Escherichia coli

0.045

-

ubiquinol-1

mutant enzyme Q82A, at pH 7.0 and 25°C

Escherichia coli

0.05

-

ubiquinol-1

wild type enzyme, at pH 7.0 and 25°C

Escherichia coli

0.072

-

ubiquinol-1

mutant enzyme F93Y, at pH 7.0 and 25°C; mutant enzyme Q101N, at pH 7.0 and 25°C

Escherichia coli

0.091

-

ubiquinol-1

mutant enzyme F93A, at pH 7.0 and 25°C

Escherichia coli

0.1

-

ubiquinol-1

mutant enzyme N157V, at pH 7.0 and 25°C

Escherichia coli

0.124

-

ubiquinol-1

mutant enzyme Q101E, at pH 7.0 and 25°C

Escherichia coli

0.141

-

ubiquinol-1

mutant enzyme Q101L, at pH 7.0 and 25°C

Escherichia coli

0.2

-

ubiquinol-1

mutant enzyme Q101T, at pH 7.0 and 25°C

Escherichia coli

Metals/Ions

Commentary

Organism

Structure

Cu2+

contains copper

Escherichia coli

Cofactor

Commentary

Organism

Structure

heme

-

Escherichia coli

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

0.000005

-

AC1-10

wild type enzyme, at pH 7.0 and 25°C

Escherichia coli

0.0007

-

2-n-heptyl-4-hydroxyquinoline N-oxide

wild type enzyme, at pH 7.0 and 25°C

Escherichia coli

Cofactor

Commentary

Organism

Structure

heme

-

Escherichia coli

Inhibitors

Commentary

Organism

Structure

2-n-heptyl-4-hydroxyquinoline N-oxide

-

Escherichia coli

AC1-10

-

Escherichia coli

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

0.000005

-

AC1-10

wild type enzyme, at pH 7.0 and 25°C

Escherichia coli

0.0007

-

2-n-heptyl-4-hydroxyquinoline N-oxide

wild type enzyme, at pH 7.0 and 25°C

Escherichia coli

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.016

-

ubiquinol-1

mutant enzyme D75E, at pH 7.0 and 25°C

Escherichia coli

0.024

-

ubiquinol-1

mutant enzyme Q101M, at pH 7.0 and 25°C

Escherichia coli

0.03

-

ubiquinol-1

mutant enzyme L160W, at pH 7.0 and 25°C; mutant enzyme Q101A, at pH 7.0 and 25°C

Escherichia coli

0.045

-

ubiquinol-1

mutant enzyme Q82A, at pH 7.0 and 25°C

Escherichia coli

0.05

-

ubiquinol-1

wild type enzyme, at pH 7.0 and 25°C

Escherichia coli

0.072

-

ubiquinol-1

mutant enzyme F93Y, at pH 7.0 and 25°C; mutant enzyme Q101N, at pH 7.0 and 25°C

Escherichia coli

0.091

-

ubiquinol-1

mutant enzyme F93A, at pH 7.0 and 25°C

Escherichia coli

0.1

-

ubiquinol-1

mutant enzyme N157V, at pH 7.0 and 25°C

Escherichia coli

0.124

-

ubiquinol-1

mutant enzyme Q101E, at pH 7.0 and 25°C

Escherichia coli

0.141

-

ubiquinol-1

mutant enzyme Q101L, at pH 7.0 and 25°C

Escherichia coli

0.2

-

ubiquinol-1

mutant enzyme Q101T, at pH 7.0 and 25°C

Escherichia coli

Metals/Ions

Commentary

Organism

Structure

Cu2+

contains copper

Escherichia coli

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

741616

Moosa

Susceptibility of Mycobacteri ...

Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv

Antimicrob. Agents Chemother.

61

e01338-17

2017

-

-

-

-

-

-

-

-

-

-

-

-

-

153

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

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-

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-

-

-

-

-

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-

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-

-

-

-

-

-

-

-

-

-

-

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-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

741988

Choi

Searching for the low affinit ...

Escherichia coli

Biochim. Biophys. Acta

1858

366-370

2017

-

-

1

-

19

-

-

13

-

1

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

1

1

-

19

-

-

-

-

13

-

1

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

741989

Li

Effects of membrane curvature ...

Escherichia coli

Biochim. Biophys. Acta

1858

763-770

2017

-

-

1

-

1

-

-

-

1

-

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1

-

2

-

-

1

-

-

-

-

-

1

-

-

-

-

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-

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

1

-

-

1

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

742647

Corbett

Listeria monocytogenes has bo ...

Listeria monocytogenes

Infect. Immun.

FEHLT

e00354-17

2017

-

-

-

-

-

-

-

-

-

-

-

1

-

5

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

742754

Choi

Location of the substrate bin ...

Escherichia coli

J. Am. Chem. Soc.

139

8346-8354

2017

-

-

1

-

15

-

2

11

-

1

-

1

-

2

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

1

2

-

-

-

-

1

1

-

15

-

-

2

2

11

-

1

-

1

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

741935

Sun

Q-band electron-nuclear doubl ...

Escherichia coli

Biochemistry

55

5714-5725

2016

-

-

1

-

-

-

-

-

1

-

-

1

-

2

-

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

743301

Korshunov

The cytochrome bd oxidase of ...

Escherichia coli

Mol. Microbiol.

101

62-77

2016

-

-

-

-

-

-

1

-

-

-

-

1

-

2

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

743596

Leclerc

The cytochrome bd oxidase of ...

Porphyromonas gingivalis, Porphyromonas gingivalis ATCC 33277

PLoS ONE

10

e0143808

2015

-

-

-

-

-

-

-

-

-

-

-

-

-

14

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

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-

-

-

-

-

-

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-

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-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

743792

Lu

The cytochrome bd-type quinol ...

Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc(2)155

Sci. Rep.

5

10333

2015

-

-

-

-

-

-

-

-

-

-

-

2

-

10

-

-

-

-

-

-

-

-

2

-

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-

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-

-

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-

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-

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-

-

-

2

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

741957

Giuffre

Cytochrome bd oxidase and bac ...

Escherichia coli

Biochim. Biophys. Acta

1837

1178-1187

2014

-

-

-

-

-

-

1

-

1

-

-

1

-

3

-

-

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

1

-

-

1

-

-

-

1

-

-

-

1

1

-

-

1

-

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1

-

-

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

742510

Hoeser

Subunit CydX of Escherichia c ...

Escherichia coli

FEBS Lett.

588

1537-1541

2014

-

-

1

-

-

-

-

-

1

-

1

1

-

2

-

-

1

-

-

-

-

-

1

1

-

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-

-

-

-

1

-

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-

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-

1

1

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-

1

-

1

1

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-

-

1

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

742946

Liu

Improved poly(3-hydroxybutyra ...

Escherichia coli, Escherichia coli JM109

J. Biotechnol.

192

170-176

2014

-

1

-

-

-

-

-

-

-

-

-

2

-

32

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

725289

Richhardt

Evidence for a key role of cyt ...

Gluconobacter oxydans

J. Bacteriol.

195

4210-4220

2013

-

-

-

-

1

-

-

-

2

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

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-

1

-

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-

-

2

-

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-

-

-

-

-

-

-

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-

-

-

-

-

-

-

-

-

-

-

-

3

3

-

-

-

723938

Yildiz

Cell-free synthesis of cytochr ...

Escherichia coli

Anal. Biochem.

423

39-45

2012

-

-

1

-

-

-

1

-

1

-

-

-

-

1

-

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

714331

Egawa

Differential effects of glutam ...

Escherichia coli, Escherichia coli C43 (DE3)

Biochim. Biophys. Acta

1807

1342-1348

2011

-

-

-

-

1

-

-

-

-

-

-

-

-

4

-

-

1

-

-

-

-

-

2

-

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-

-

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-

-

-

1

-

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-

1

-

1

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-

-

1

-

-

-

-

2

-

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-

-

-

-

-

-

714308

Yap

The quinone-binding sites of t ...

Escherichia coli

Biochim. Biophys. Acta

1797

1924-1932

2010

-

-

-

-

12

-

2

4

1

1

-

-

-

2

-

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

2

4

-

-

-

-

-

2

-

12

-

-

2

4

4

1

1

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

715509

Shepherd

Compensations for diminished t ...

Escherichia coli, Escherichia coli BW25113

J. Biol. Chem.

285

18464-18472

2010

-

-

-

-

-

-

-

-

-

-

-

-

-

29

-

-

-

-

-

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-

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1

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1

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-

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-

-

-

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2

2

-

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-

714169

Egawa

Communication between R481 and ...

Escherichia coli

Biochemistry

48

12113-12124

2009

-

-

-

-

2

-

-

-

-

1

-

-

-

2

-

-

1

-

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-

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1

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1

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2

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1

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1

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-

-

1

1

-

-

-

716735

Belevich

Time-resolved electrometric an ...

Escherichia coli, Escherichia coli GO105

Proc. Natl. Acad. Sci. USA

102

3657-3662

2005

-

-

-

-

1

-

-

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-

-

-

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-

4

-

-

1

-

-

-

-

-

2

-

-

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-

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-

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1

-

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1

-

1

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1

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2

-

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-

-

-

-

-

715458

Park

Vitreoscilla hemoglobin binds ...

Escherichia coli, Escherichia coli JM103, Pseudomonas aeruginosa, Vitreoscilla sp.

J. Biol. Chem.

277

33334-33337

2002

-

-

-

-

-

-

-

-

3

-

-

-

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9

-

-

-

-

-

-

-

-

4

-

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-

-

-

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-

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-

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-

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-

-

-

3

-

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-

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-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

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-

-

-

713612

Abramson

Purification, crystallization ...

Escherichia coli, Escherichia coli GO105

Acta Crystallogr. Sect. D

56

1076-1078

2000

-

-

-

1

-

-

-

-

-

-

-

-

-

5

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

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-

-

-

1

-

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-

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-

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-

1

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-

1

-

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-

-

-

-

-

-

-

-

-

716311

Abramson

The structure of the ubiquinol ...

Escherichia coli

Nat. Struct. Biol.

7

910-917

2000

-

-

-

1

5

-

-

5

1

1

-

-

-

2

-

-

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

1

5

-

-

-

-

5

1

1

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

1

1

-

-

-

715003

Mogi

Role of a bound ubiquinone on ...

Escherichia coli, Escherichia coli GO103/pHN3795-1

FEBS Lett.

457

223-226

1999

-

-

-

-

-

-

-

-

-

-

-

-

-

3

-

-

1

-

-

-

-

-

2

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

714141

Mogi

Substitutions of conserved aro ...

Escherichia coli, Escherichia coli GO103/pMFO2

Biochemistry

37

1632-1639

1998

-

-

-

-

16

-

-

-

1

1

-

-

-

3

-

-

-

-

-

-

-

-

2

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

16

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

2

1

-

-

-

-

-

-

-

-

-

1

1

-

-

-

714139

Ek

Oxidation of ubiquinol by cyto ...

Escherichia coli

Biochemistry

36

5425-5431

1997

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

715000

Tsubaki

Glutamate-286 mutants of cytoc ...

Escherichia coli, Escherichia coli GO103

FEBS Lett.

416

247-250

1997

-

-

-

-

3

-

1

-

1

1

-

-

-

4

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

3

-

-

1

-

-

1

1

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

715399

Kawasaki

Substitutions of charged amino ...

Escherichia coli, Escherichia coli ST4676

J. Biochem.

122

422-429

1997

-

-

-

-

15

-

-

-

2

1

-

-

-

3

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

15

-

-

-

-

-

2

1

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

714135

Garcia-Horsman

Proton transfer in cytochrome ...

Escherichia coli

Biochemistry

34

4428-4433

1995

-

-

1

-

21

-

-

-

1

1

-

-

-

2

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

1

1

-

21

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

714997

Orii

Facilitated intramolecular ele ...

Escherichia coli, Escherichia coli GO103

FEBS Lett.

352

151-154

1994

-

-

-

-

-

-

-

-

-

1

-

-

-

3

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

714130

Thomas

Substitution of asparagine for ...

Escherichia coli, Escherichia coli GL101

Biochemistry

32

10923-10928

1993

-

-

-

-

7

-

-

-

1

-

-

-

-

6

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

7

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

714131

Thomas

Site-directed mutagenesis of h ...

Escherichia coli, Escherichia coli GL101

Biochemistry

32

11173-11180

1993

-

-

1

-

10

-

-

-

1

-

-

-

-

4

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

1

-

-

-

-

-

1

1

-

10

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

714132

Calhoun

Site-directed mutants of the c ...

Escherichia coli, Escherichia coli GLlOl

Biochemistry

32

11524-11529

1993

-

-

-

-

6

-

-

-

-

1

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

6

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

714285

Miyoshi-Akiyama

Purification and properties of ...

Vibrio alginolyticus

Biochim. Biophys. Acta

1141

283-287

1993

6

-

-

-

-

-

3

-

1

1

3

-

-

4

-

-

1

-

-

-

-

-

4

1

-

-

-

-

-

-

-

2

-

-

3

6

-

-

2

-

-

-

3

3

-

-

1

1

3

-

-

-

-

1

-

-

-

-

4

1

-

-

-

-

-

-

-

-

-

1

1

-

-

-

714286

Bolgiano

Reactions of the membrane-boun ...

Escherichia coli, Escherichia coli RG145

Biochim. Biophys. Acta

1141

95-104

1993

-

-

-

-

-

-

-

-

1

-

-

-

-

5

-

-

-

-

-

-

-

-

2

-

-

-

-

2

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

714129

Minghetti

Modified, large-scale purifica ...

Escherichia coli, Escherichia coli RG145

Biochemistry

31

6917-6924

1992

-

-

-

-

-

-

-

-

2

3

4

-

-

5

-

-

1

-

-

-

1

-

4

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

2

3

4

-

-

-

-

1

-

-

1

-

4

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

714885

Salerno

Orientation of the haems of th ...

Escherichia coli, Escherichia coli RG145

Eur. J. Biochem.

198

789-792

1991

-

-

-

-

-

-

-

-

1

-

-

-

-

5

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

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-