Literature summary for 7.1.1.8 extracted from

Van Loon, A.P.G.M.; Kreike, J.; de Ronde, A.; van der Horst, G.T.; Gasser, S.M.; Grivell, L.A.
Biosynthesis of the ubiquinol-cytochrome c reductase complex in yeast. Characterization of precursor forms of the 44-kDa, 40-kDa and 17-kDa subunits and identification of individual messenger RNAs for these and other imported subunits of the complex (1983), Eur. J. Biochem., 135, 457-463.

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	subunits specified by nuclear genes and sythesized in the cytoplasm	Saccharomyces cerevisiae	5737	-
cytoplasm	subunits specified by nuclear genes and sythesized in the cytoplasm	Neurospora crassa	5737	-
mitochondrion	-	Saccharomyces cerevisiae	5739	-
mitochondrion	-	Neurospora crassa	5739	-
spheroplast	-	Saccharomyces cerevisiae	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
11000	-	1 * 44000 + 1 * 40000 + 2 * 31000 + 1 * 25000 + 1 * 17000 + 1 * 14000 + 1 * 11000, SDS-PAGE	Saccharomyces cerevisiae
11200	-	1 * 50000 + 1 * 45000 + 2 * 31000 + 1 * 25000 + 1 * 14000 + 1 * 11500 + 1 * 11200, SDS-PAGE	Neurospora crassa
11500	-	1 * 50000 + 1 * 45000 + 2 * 31000 + 1 * 25000 + 1 * 14000 + 1 * 11500 + 1 * 11200, SDS-PAGE	Neurospora crassa
14000	-	1 * 50000 + 1 * 45000 + 2 * 31000 + 1 * 25000 + 1 * 14000 + 1 * 11500 + 1 * 11200, SDS-PAGE	Neurospora crassa
14000	-	1 * 44000 + 1 * 40000 + 2 * 31000 + 1 * 25000 + 1 * 17000 + 1 * 14000 + 1 * 11000, SDS-PAGE	Saccharomyces cerevisiae
17000	-	1 * 44000 + 1 * 40000 + 2 * 31000 + 1 * 25000 + 1 * 17000 + 1 * 14000 + 1 * 11000, SDS-PAGE	Saccharomyces cerevisiae
25000	-	iron-sulfur protein [Fe2-S2], SDS-PAGE	Saccharomyces cerevisiae
25000	-	iron-sulfur protein [Fe2-S2], SDS-PAGE	Neurospora crassa
31000	-	cytochrome c and cytochrome b, SDS-PAGE	Saccharomyces cerevisiae
31000	-	cytochrome c and cytochrome b, SDS-PAGE	Neurospora crassa
40000	-	1 * 44000 + 1 * 40000 + 2 * 31000 + 1 * 25000 + 1 * 17000 + 1 * 14000 + 1 * 11000, SDS-PAGE	Saccharomyces cerevisiae
44000	-	core protein, SDS-PAGE	Saccharomyces cerevisiae
45000	-	1 * 50000 + 1 * 45000 + 2 * 31000 + 1 * 25000 + 1 * 14000 + 1 * 11500 + 1 * 11200, SDS-PAGE	Neurospora crassa
50000	-	1 * 50000 + 1 * 45000 + 2 * 31000 + 1 * 25000 + 1 * 14000 + 1 * 11500 + 1 * 11200, SDS-PAGE	Neurospora crassa

Organism

Organism	UniProt	Comment	Textmining
Neurospora crassa	-	-	-
Saccharomyces cerevisiae	-	-	-
Saccharomyces pastorianus	-	derived from Saccharomyces cerevisiae KL14-4A	-
Saccharomyces pastorianus NCYC74	-	derived from Saccharomyces cerevisiae KL14-4A	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ubiquinol + 2 ferricytochrome c	-	Saccharomyces pastorianus	ubiquinone + 2 ferrocytochrome c + 2 H+	-	?
ubiquinol + 2 ferricytochrome c	-	Saccharomyces cerevisiae	ubiquinone + 2 ferrocytochrome c + 2 H+	-	?
ubiquinol + 2 ferricytochrome c	-	Neurospora crassa	ubiquinone + 2 ferrocytochrome c + 2 H+	-	?
ubiquinol + 2 ferricytochrome c	-	Saccharomyces pastorianus NCYC74	ubiquinone + 2 ferrocytochrome c + 2 H+	-	?

Subunits

Subunits	Comment	Organism
heptamer	-	Saccharomyces cerevisiae
heptamer	-	Neurospora crassa
octamer	1 * 50000 + 1 * 45000 + 2 * 31000 + 1 * 25000 + 1 * 14000 + 1 * 11500 + 1 * 11200, SDS-PAGE	Neurospora crassa
octamer	1 * 44000 + 1 * 40000 + 2 * 31000 + 1 * 25000 + 1 * 17000 + 1 * 14000 + 1 * 11000, SDS-PAGE	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)