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Literature summary for 7.1.1.8 extracted from
- Large-scale purification and characterization of a highly active four-subunit cytochrome bc1 complex from Rhodobacter sphaeroides (1990), Biochemistry, 29, 2645-2651.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dodecyl maltoside | - |
Paracoccus denitrificans |  |
| dodecyl maltoside | - |
Bos taurus | |
| dodecyl maltoside | - |
Cereibacter sphaeroides | |
| lecithin | soybean | Cereibacter sphaeroides | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| fbc operon cloned and sequenced | Rhodobacter capsulatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | - |
Cereibacter sphaeroides | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chromatophore | inner surface of the chromatophore membrane | Cereibacter sphaeroides | - |
- |
| mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron-sulfur cluster | - |
Bos taurus | |
| Iron-sulfur cluster | - |
Cereibacter sphaeroides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
1 * 40000 + 1 * 34000 + 1 * 24000 + 1 * 14000, SDS-PAGE | Cereibacter sphaeroides |
| 24000 | - |
1 * 40000 + 1 * 34000 + 1 * 24000 + 1 * 14000, SDS-PAGE | Cereibacter sphaeroides |
| 34000 | - |
1 * 40000 + 1 * 34000 + 1 * 24000 + 1 * 14000, SDS-PAGE | Cereibacter sphaeroides |
| 40000 | - |
1 * 40000 + 1 * 34000 + 1 * 24000 + 1 * 14000, SDS-PAGE | Cereibacter sphaeroides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ubiquinone-10 + ferricytochrome c | Paracoccus denitrificans | - |
ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | Bos taurus | - |
ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | Cereibacter sphaeroides | - |
ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | Rhodobacter capsulatus | - |
ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | Bacillus sp. (in: Bacteria) | - |
ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Cereibacter sphaeroides | - |
- |
- |
| Paracoccus denitrificans | - |
- |
- |
| Rhodobacter capsulatus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phospholipoprotein | - |
Paracoccus denitrificans |
| phospholipoprotein | - |
Bos taurus |
| phospholipoprotein | - |
Cereibacter sphaeroides |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cereibacter sphaeroides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 50 mM Tris-HCl buffer, pH 8.0, 200 mM NaCl, 0.1 mg/ml dodecylmaltoside, 50% glycerol | Cereibacter sphaeroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| quinol + 2 horse heart ferricytochrome c1 | - |
Cereibacter sphaeroides | quinone + 2 horse heart ferrocytochrome c1 + H+ [side 2] | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Paracoccus denitrificans | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Bos taurus | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Cereibacter sphaeroides | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Rhodobacter capsulatus | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Bacillus sp. (in: Bacteria) | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinol + horse heart cytochrome c | - |
Cereibacter sphaeroides | ? | - |
? | |
| ubiquinone-10 + ferricytochrome c | - |
Paracoccus denitrificans | ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | - |
Bos taurus | ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | - |
Cereibacter sphaeroides | ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | - |
Rhodobacter capsulatus | ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | - |
Bacillus sp. (in: Bacteria) | ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Rhodobacter capsulatus |
| tetramer | 1 * 40000 + 1 * 34000 + 1 * 24000 + 1 * 14000, SDS-PAGE | Cereibacter sphaeroides |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.17 | 5 | 2 horse heart ferricytochrome c1 | - |
Cereibacter sphaeroides | |
| 25 | 35 | cytochrome c | - |
Cereibacter sphaeroides | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme b | prosthetic group | Cereibacter sphaeroides | |
| heme c | prosthetic group | Cereibacter sphaeroides | |
| Phospholipid | - |
Bos taurus | |
| Phospholipid | prosthetic group | Cereibacter sphaeroides | |
| semiquinone | antimycin-sensitive prosthetic group | Cereibacter sphaeroides | |
| ubiquinone | - |
Bos taurus | |
| ubiquinone | prosthetic group | Cereibacter sphaeroides | |
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