Cloned (Comment) | Organism |
---|---|
cytochrome b gene, DNA sequence determination of wild-type and mutant strains | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
G37D | site-directed mutagenesis, the mutation is located on exon 1, the mutant is less sensitive to ilicicolin H, compared to the wild-type, and shows normal growth | Saccharomyces cerevisiae |
G37S | site-directed mutagenesis, the mutation is located on exon 1, the mutant is less sensitive to ilicicolin H, compared to the wild-type, and shows impaired growth | Saccharomyces cerevisiae |
L198F | site-directed mutagenesis, the mutation is located on exon 4, the mutant is less sensitive to ilicicolin H, compared to the wild-type, and shows normal growth | Saccharomyces cerevisiae |
additional information | construction of several mutants, some of which are resistant to center N site enzyme inhibitors, such as ilicicolin H or antimycin, genomic structure of mutants, effects of the cytochrome b mutations on respiratory growth and cross-resistance to other Qn site inhibitors, overview | Saccharomyces cerevisiae |
Q22E | site-directed mutagenesis, the mutation is located on exon 1, the mutant is resistant to ilicicolin H and shows normal growth | Saccharomyces cerevisiae |
Q22T | site-directed mutagenesis, the mutation is located on exon 1, the mutant is resistant to ilicicolin H and shows normal growth | Saccharomyces cerevisiae |
S20L | site-directed mutagenesis, the mutation is located on exon 1, the mutant is resistant to ilicicolin H and shows impaired growth | Saccharomyces cerevisiae |
S20T | site-directed mutagenesis, the mutation is located on exon 1, the mutant is resistant to ilicicolin H and shows normal growth | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Antimycin | a Qn site inhibitor | Saccharomyces cerevisiae | |
funiculosin | a Qn site inhibitor | Saccharomyces cerevisiae | |
ilicicolin H | a Qn site inhibitor, a 5-(4-hydroxyphenyl)-alpha-pyridone with a decalin ring system, wild-type enzyme IC50: 12 nM | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Saccharomyces cerevisiae | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquinol + ferricytochrome c | Saccharomyces cerevisiae | the cytochrome bc1 complex resides in the inner membrane of mitochondria and transfers electrons from ubiquinol to cytochrome c, this electron transfer is coupled to the translocation of protons across the membrane by the protonmotive Q cycle mechanism, this mechanism topographically separates reduction of quinone and reoxidation of quinol at sites on opposite sites of the membrane, referred to as center N, Qn site, and center P, Qp site, respectively | ubiquinone + ferrocytochrome c | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
wild-type strain W303-1B | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
decylubiquinol + ferricytochrome c | - |
Saccharomyces cerevisiae | decylubiquinone + ferrocytochrome c | - |
? | |
ubiquinol + ferricytochrome c | - |
Saccharomyces cerevisiae | ubiquinone + ferrocytochrome c | - |
? | |
ubiquinol + ferricytochrome c | the cytochrome bc1 complex resides in the inner membrane of mitochondria and transfers electrons from ubiquinol to cytochrome c, this electron transfer is coupled to the translocation of protons across the membrane by the protonmotive Q cycle mechanism, this mechanism topographically separates reduction of quinone and reoxidation of quinol at sites on opposite sites of the membrane, referred to as center N, Qn site, and center P, Qp site, respectively | Saccharomyces cerevisiae | ubiquinone + ferrocytochrome c | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure of yeast cytochrome b and a view of center N, overview | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
cytochrome bc1 complex | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
65 | - |
decylubiquinol | pH 7.0, wild-type enzyme | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Saccharomyces cerevisiae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000012 | - |
a Qn site inhibitor, a 5-(4-hydroxyphenyl)-alpha-pyridone with a decalin ring system, wild-type enzyme IC50: 12 nM | Saccharomyces cerevisiae | ilicicolin H |